基于UHPLC-LTQ-Orbitrap-MS、GC-MS和UHPLC-QQQ-MS/MS技术的散寒化湿方化学成分轮廓分析  

Component profile analysis of Sanhan Huashi Formula based on UHPLC-LTQ-Orbitrap-MS,GC-MS,and UHPLC-QQQ-MS/MS

作　　者：王梦晓 姚晨思 赵海誉[1] 王维皓[1] 张艳 罗珂珂 司南[1] 边宝林[1] 李涛[1,3] 魏晓露[1] 田孟尧 周严严[1] 王宏洁[1] 李敏[2] WANG Meng-xiao;YAO Chen-si;ZHAO Hai-yu;WANG Wei-hao;ZHANG Yan;LUO Ke-ke;SI Nan;BIAN Bao-lin;LI Tao;WEI Xiao-lu;TIAN Meng-yao;ZHOU Yan-yan;WANG Hong-jie;LI Min(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Guang′anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment of Major Diseases,Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国中医科学院广安门医院,北京100053 [3]中国中医科学院医学实验中心,北京市中医药防治重大疾病基础研究重点实验室,北京100700

出　　处：《中国中药杂志》2023年第22期6066-6074,共9页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划专项(2022YFC0868300,2020YFE0201100)。

摘　　要：该研究采用定性定量质谱技术全面分析散寒化湿方药效成分,为阐明其药效物质基础奠定依据。采用UHPLC-LTQ-Orbitrap-MS与GC-MS技术分析鉴定了散寒化湿方中挥发性和非挥发性成分;采用UHPLC-QQQ-MS/MS技术同时测定散寒化湿方27个主要药效成分的含量。结果显示从散寒化湿方中分析鉴定了308个主要化学成分,其中60个化合物经对照品比对,主要包括生物碱类、黄酮类、香豆素类、三萜皂苷类、氨基酸类和核苷类等。GC-MS技术初步鉴定了52个挥发性化合物,其中γ-桉叶醇、β-桉叶醇为主要成分。液质定量结果表明,27个活性成分线性关系良好(r>0.99),所建立的方法稳定,操作简单,结果可靠,其中苦杏仁苷、紫花前胡苷、槟榔碱、麻黄碱和伪麻黄碱含量较高,可能是发挥作用的主要药效物质。综上所述,该研究系统、全面地表征了散寒化湿方药味所含主要化成分及模式,为散寒化湿方的药效机制及临床应用提供依据。This study comprehensively analyzed the active components of Sanhan Huashi Formula using qualitative and quantitative mass spectrometry techniques,laying the foundation for understanding its pharmacological substance basis.UHPLC-LTQ-Orbitrap-MS and GC-MS technologies were used to analyze and identify the volatile and non-volatile components in Sanhan Huashi Formula.UHPLC-QQQ-MS/MS technology was used to simultaneously determine the content of 27 major active components in the formula.The results showed that 308 major chemical components were identified in Sanhan Huashi Formula,among which 60 compounds were identified by comparing with reference standards,mainly including alkaloids,flavonoids,coumarins,triterpenoid saponins,amino acids,and nucleosides.GC-MS technology preliminarily identified 52 volatile compounds,with γ-eudesmol and β-eudesmol as the main components.The quantitative results demonstrated good linearity(r>0.99) for the 27 active components,indicating the stability,simplicity,and reliability of the established method.Among them,amygdalin,nodakenin,arecoline,ephedrine,and pseudoephedrine had relatively high content and were presumably the main pharmacologically active substances.In conclusion,this study systematically and comprehensively characterized the major chemical components and patterns in Sanhan Huashi Formula,providing a basis for understanding its pharmacological mechanisms and clinical applications.

关 键 词：散寒化湿方 UHPLC-LTQ-Orbitrap-MS GC-MS 活性成分 UHPLC-QQQ-MS/MS 含量测定 

分 类 号：R284.1[医药卫生—中药学]