理肺散化学成分研究  

Chemical constituents from whole herb of Hedyotis scandens

作　　者：王钰君 黄居敏 文淳 周子硕 冯桥桥 胡昌华[1] 周培富[2] 尹国平 WANG Yu-jun;HUANG Ju-min;WEN Chun;ZHOU Zi-shuo;FENG Qiao-qiao;HU Chang-hua;ZHOU Pei-fu;YIN Guo-ping(Engineering Research Center of Coptis Development and Utilization(Ministry of Education),College of Pharmaceutical Sciences,Southwest University,Chongqing 400715,China;Key Laboratory of Guizhou Ethnic Medicine Resource Development and Utilization in Guizhou Minzu University,State Ethnic Affairs Commission,School of Ethnic-Minority Medicine,Guizhou Minzu University,Guiyang 550025,China)

机构地区：[1]西南大学药学院黄连开发与利用教育部工程研究中心,重庆400715 [2]贵州民族大学民族医药学院,国家民委贵州省少数民族医药资源开发与利用重点实验室,贵州贵阳550025

出　　处：《中国中药杂志》2023年第22期6082-6087,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(82003629);重庆市自然科学基金项目(cstc2021jcyj-msxmX0975);大学生创新创业训练计划项目(202110635104,202210635021)。

摘　　要：对理肺散水提取部位进行化学成分研究,采用硅胶、ODS、MCI等柱色谱以及制备HPLC等分离手段,利用NMR、HR-ESI-MS等结构鉴定方法,从中分离鉴定了13个化合物,分别为methyl 4-benzoyloxy-3-methoxybenzeneacetate (1)、4-benzoyloxy-3-methoxybenzeneacetic acid (2)、3-(4-hydroxy-3-methoxyphenyl)-propanoic acid (3)、水杨酸(4)、3-hydroxy-4-methoxypyridine (5)、丁香酸(6)、对羟基桂皮酸(7)、(R)-6-methyl-4,6-bis(4-methylpent-3-enyl)cyclohexa-1,3-dienecarbaldehyde (8)、1,2-bis(4-hydroxy-3-methoxyphenyl)-1,3-propanediol (9)、1H-indole-3-carboxaldehyde (10)、异东莨菪内酯(11)、syringaresinol (12)、pinoresinol (13)。其中,化合物1和2为2个新的酚酸类化合物,化合物3~5、8~11、13为首次从耳草属中分离发现,化合物6、7、12为首次从理肺散中分离发现。活性测试结果显示,化合物1和10对耻垢分枝杆菌具有一定抑制活性,其MIC_(50)分别为58.5、33.3μg·mL^(-1)。This study aimed to investigate the chemical constituents in the water extract of the whole herb of Hedyotis scandens by silica gel,ODS,and MCI column chromatographies together with preparative high-performance liquid chromatography(HPLC).The structures of isolated constituents were identified by NMR,HR-ESI-MS,etc.Thirteen compounds were isolated and identified as methyl 4-benzoyloxy-3-methoxybenzeneacetate(1),4-benzoyloxy-3-methoxybenzeneacetic acid(2),3-(4-hydroxy-3-methoxyphenyl)-propanoic acid(3),salicylic acid(4),3-hydroxy-4-methoxypyridine(5),syringic acid(6),hydroxycinnamic acid(7),(R)-6-methyl-4,6-bis(4-methylpent-3-enyl)cyclohexa-1,3-dienecarbaldehyde(8),1,2-bis(4-hydroxy-3-methoxyphenyl)-1,3-propanediol(9),1H-indole-3-carboxaldehyde(10),isoscopoletin(11),syringaresinol(12),and pinoresinol(13).Among them,compounds 1 and 2 were new phenolic acid compounds,compounds 3-5,8-11,and 13 were isolated from this genus for the first time,and compounds 6,7,and 12 were obtained from H.scandens for the first time.The activity test showed that compounds 1 and 10 had a certain inhibitory effect on Mycobacterium smegmatis,with MIC_(50) values of 58.5 and 33.3 μg·mL^(-1),respectively.

关 键 词：理肺散 耳草属 化学成分 抗菌活性 

分 类 号：R284.1[医药卫生—中药学]