HPLC法测定胃镜消泡剂中盐酸利多卡因含量  

Determination of Content of Lidocaine Hydrochloride in Gastroscope Defoamer by HPLC

作　　者：殷敏 李秋璐 董自波 付免 YIN Min;LI Qiu-lu;DONG Zi-bo;FU Mian(Pharmacy School,Jiangsu Ocean University,Lianyungang Jiangsu 222000,China)

机构地区：[1]江苏海洋大学药学院,江苏连云港222000

出　　处：《当代化工》2023年第11期2698-2701,2766,共5页Contemporary Chemical Industry

基　　金：江苏省高等学校自然科学面上项目,含非蛋白质氨基酸的KLA类似物抗肿瘤活性及机制研究(项目编号:21KJB350019);江苏省海洋药物活性分子筛选重点实验室开放课题,新型诱导肿瘤凋亡肽的设计、合成与活性评估(项目编号:HY202003);江苏海洋大学人才引进科研基金项目,博士科研启动费(项目编号:KQ20049)。

摘　　要：采用高效液相色谱(HPLC)法测定胃镜消泡剂中盐酸利多卡因的含量。色谱柱为Kromasil C_(18)(250 mm×4.6 mm,5μm),流动相为磷酸盐缓冲液(取1 mol·L^(-1)磷酸二氢钠溶液1.3 mL和0.5 mol·L^(-1)磷酸氢二钠溶液32.5 mL,置1000 mL容量瓶中,加水稀释至刻度,摇匀)-乙腈(50∶50,磷酸调节pH至8.0),流速为1.0 mL·min^(-1),检测波长为254 nm,柱温为30℃。盐酸利多卡因在0.01887~0.37740 mg·mL^(-1)范围内线性关系良好(r=0.9996),平均回收率为99.38%,RSD为0.57%。该方法简便、准确,可用于胃镜消泡剂中盐酸利多卡因的含量控制。The lidocaine hydrochloride content in gastroscope defoamer was detectedby using HPLC,with the column of Kromasil C_(18)(250 mm×4.6 mm,5μm),the mobile phase phosphate buffer(1 mol·L^(-1)sodium dihydrogen phosphate solution 1.3 mL and 0.5 mol·L^(-1)disodium hydrogen phosphate solution 32.5 mL in a 1000 mL flask were diluted with water to the scale)-acetonitrile(50∶50,adjusted to pH 8.0 with phosphoric acid).The flow rate was 1.0mL·min^(-1),the detection wavelength was at 254 nm,the column temperature was 30℃.The results showed that lidocaine hydrochloride had a good linear relationship in the range of 0.01887~0.37740 mg·mL^(-1)(r=0.9996).The average recovery was 99.38%with RSD 0.57%.The established method is simple and accurate,and could be used as a quality control method for gastroscope defoamer.

关 键 词：高效液相色谱法 胃镜消泡剂 盐酸利多卡因 含量 方法学验证 

分 类 号：TQ460.72[医药卫生—药物分析学]