HBV DNA阳性献血者感染标志物定量分析  

Quantitative analysis of infection markers in HBV DNA-positive blood donors

作　　者：洪淑芳 吴昕 杜晓明 钱江 HONG Shufang;WU Xin;DU Xiaoming(Department of Laboratory,Jinhua Central Blood Station,Jinhua 321000,China)

机构地区：[1]金华市中心血站输血研究所,浙江金华321000

出　　处：《全科医学临床与教育》2023年第12期1083-1085,共3页Clinical Education of General Practice

基　　金：浙江省血液安全研究重点实验室开放课题资助项目(2021KF002)。

摘　　要：目的探究乙型肝炎病毒核酸(HBV DNA)阳性献血者感染标志物含量特征。方法收集2019年9月至2021年5月金华市中心血站HBV DNA阳性献血者标本,运用时间分辨荧光免疫分析(TRFIA)定量检测乙肝五项和实时荧光PCR技术定量检测HBV DNA,并按照HBsAg阴性和HBsAg阳性进行分组比较感染标志物含量。结果共检测46324例标本,发现HBV DNA阳性124例,阳性率0.27%,平均HBV DNA浓度(1.82±0.94)lgIU/mL。其中,HBsAg阴性组64例、HBsAg阳性组60例。HBsAg阴性组HBcAb阳性率为93.75%,主要以HBeAb(+)+HBcAb(+)及单独HBcAb(+)血清学模式为主,HBcAb平均浓度9.01(6.10,11.51)PEIU/mL,HBsAb平均浓度为3.18(1.06,14.86)mIU/mL,HBV DNA平均浓度为1.33(0.90,1.70)lgIU/mL。HBsAg阳性组HBcAb阳性率为100%,主要以HBeAb(+)+HBcAb(+)模式为主,HBcAb平均浓度14.85(9.90,18.48)PEIU/m,HBsAb平均浓度为0.80(0.17,1.46)mIU/mL,HBV DNA平均浓度为2.15(1.70,3.06)lgIU/mL。两组HBcAb浓度、HBsAb浓度、HBV DNA定量比较,差异均有统计学意义(Z分别=-4.87、-5.43、-6.67,P均<0.05)。结论HBV DNA阳性献血者中,HBsAg阴性率略高于HBsAg阳性率,HBsAg阳性献血者的HBcAb浓度和HBV DNA浓度高于HBsAg阴性献血者,核酸浓度与HBcAb浓度呈正相关关系。Objective To investigate the characteristics of infection markers in nucleic acid positive hepatitis B virus blood donors.Methods Specimens of hepatitis B virus nucleic acid-positive blood donors were collected from Jinhua central blood station from September 2019 to May 2021,and further quantified by time-resolved fluorescence immunoas⁃say(TRFIA)for hepatitis B five parameters and real-time fluorescence PCR for HBV DNA,and they were devided into HBsAg-negative and HBsAg-positive groups to compare these indexes.Results A total of 46324 specimens were tested and 124 cases were found to be positive for hepatitis B virus DNA,with a positive rate of 0.27%and an average HBV DNA concentration of(1.82±0.94)lgIU/mL,of which 64 cases in the HBsAg negative group and 60 cases in the HBsAg positive group.The HBcAb positivity rate in the HBsAg negative group was 93.75%,mainly based on the serological pattern of HBeAb(+)and HBcAb(+)or HBcAb(+)alone,with a mean HBcAb concentration of 9.01(6.10,11.51)PEIU/mL,HBsAb concentration of 3.18(1.06,14.86)mIU/mL and HBV DNA concentration of 1.33(0.90,1.70)lgIU/mL.In the HBsAg positive group,the HBcAb positivity rate was 100%,mainly in the HBeAb(+)+HBcAb(+)pattern,with a mean HBcAb concentration of 14.85(9.90,18.48)PEIU/m,HBsAb concentration of 0.80(0.17,1.46)mIU/mL and a mean HBV DNA concentration of 2.15(1.70,3.06)lgIU/mL.The differences in HBcAb concentration,HBsAb concentration,and HBV DNA quantification between the two groups were statistically significant(Z=-4.87,-5.43,-6.67,P<0.05).Conclusion Among the hepatitis B virus nucleic acid-positive blood donors,the percentage of HBsAg-negative was higher than that of HBsAg-positive.HBsAg-positive donors had higher HBcAb and HBV DNA concentra⁃tions than HBsAg-negative donors,and the nucleic acid concentrations were positively correlated with HBcAb concentration.

关 键 词：乙型肝炎病毒核酸 乙肝核心抗体 乙肝表面抗原 献血者 

分 类 号：R512.62[医药卫生—内科学]