熟地黄的炮制及有效成分和微量元素的检测研究  

Study on Concoction and Detection of Active Components and Trace Elements of Radix Rehmanniae Praeparata

作　　者：陈胜强 杨克培 康文利 周永昌 陶思曼 关伊辰 蒲秀瑛[1] CHEN Shengqiang;YANG Kepei;KANG Wenli;ZHOU Yongchang;TAO Siman;GUAN Yichen;PU Xiuying(Lanzhou University of Technology,Lanzhou 730050,China;Gansu Agricultural Reclamation Drug Alkali Plant Co.Ltd.,Lanzhou 730000,China)

机构地区：[1]兰州理工大学,甘肃兰州730050 [2]甘肃农垦药物碱厂有限公司,甘肃兰州730000

出　　处：《分析测试技术与仪器》2023年第4期392-399,共8页Analysis and Testing Technology and Instruments

基　　金：甘肃省科技厅重点研发计划项目(编号:20YF8FA060)。

摘　　要：炮制出熟地黄,并建立熟地黄中5种有效苷类成分的高效液相色谱(HPLC)及微量元素的电感耦合等离子体质谱(ICP-MS)的测定方法,以此优选熟地黄的最佳炮制工艺.以传统酒蒸熟地黄工艺为对照,5种有效苷类成分及微量元素的含量为评价指标,确定熟地黄不同次数炮制的最优工艺.结果发现,通过“七蒸七晒”炮制工艺所获熟地黄显著增加了其中梓醇、地黄苷D、益母草苷、毛蕊花糖苷、异毛蕊花糖苷含量以及铁、锌、铜微量元素含量,同时降低了镉、砷有害元素含量.利用HPLC和ICP-MS分别精准的检测到熟地黄中5种有效苷类成分和微量元素的含量,确定“七蒸七晒”为熟地黄最佳炮制工艺,为熟地黄炮制方法提供了科学依据.A high performance liquid chromatography(HPLC)for the determination of five effective glycosides and an inductively coupled plasma mass spectrometry(ICP-MS)for the determination of trace elements in Radix Rehmanniae Praeparata were established to optimize the processing technology of Radix Rehmanniae Praeparata.The traditional winesteaming process of Radix Rehmanniae Praeparata was used as a control,and the contents of five effective glycosides and trace elements were used as evaluation indexes to determine the optimal process for the different frequencies of Radix Rehmanniae Praeparata concoctions.The results showed that the content of Catalpol,Rehmannioside D,Leonuride,Acteoside,Isoacteoside as well as the content of trace elements of iron,zinc and copper were increased,while the content of harmful elements of cadmium and arsenic were reduced in Radix Rehmanniae Praeparata obtained by the"sevensteaming and seven-sun-drying"concocting process.Five kinds of effective glycosides and trace elements in Radix Rehmanniae Praeparata were accurately detected by HPLC and ICP-MS,respectively,and"seven-steaming and sevendrying"was determined as the best processing technology for Radix Rehmanniae Praeparata,which provided a scientific basis for the preparation of Radix Rehmanniae Praeparata.

关 键 词：熟地黄 炮制方法 高效液相色谱法 电感耦合等离子体质谱法 

分 类 号：O657.72[理学—分析化学] O657.63[理学—化学]