周8425B抗叶锈病基因LrZH84和LrZH22/Lr13在其衍生品种中的遗传解析  

Genetic analysis of Zhou 8425B leaf rust resistance gene LrZH84 and LrZH22/Lr13 in their derivative varieties

作　　者：董纯豪 李俣佳 唐建卫 郑继周 邱军 谷登斌 王道文 郑天存 殷贵鸿 DONG Chunhao;LI Yujia;TANG Jianwei;ZHENG Jizhou;QIU Jun;GU Dengbin;WANG Daowen;ZHENG Tiancun;YIN Guihong(College of Agronomy,Henan Agricultural University/State Key Laboratory of Wheat and Maize Crop Science Co-built by Henan Province and the Ministry of Agriculture and Rural Affaris/Henan Grain Crop Collaborative Innovation Center/National Wheat Engineering Technology Research Center,Zhengzhou 450046,China;Henan Fengdekang Seedlings Technology Co.,Ltd.,Zhengzhou 450001,China;The National Service Center of Agricultural Technique Popularization,Beijing 100125,China)

机构地区：[1]河南农业大学农学院,省部共建小麦玉米作物学国家重点实验室,河南粮食作物协同创新中心,国家小麦工程技术研究中心,河南郑州450046 [2]河南丰德康种业股份有限公司,河南郑州454001 [3]全国农业技术推服务推广中心,北京100125

出　　处：《河南农业大学学报》2023年第6期897-906,共10页Journal of Henan Agricultural University

基　　金：河南省重大科技专项项目(201300110800);河南省现代农业产业技术体系建设项目(HARS-22-01-G2);河南省农业良种联合攻关项目(2022010102);河南省科技研发计划联合基金项目(222103810002);2022年度河南省产业研发联合基金重大项目(225101610048);河南农业大学拔尖人才项目(30500678);国家重点研发计划项目(2021YFF1000203-13)。

摘　　要：【目的】分析小麦骨干亲本周8425B的207份衍生品种中叶锈病抗性基因LrZH84和LrZH22/Lr13的分布及遗传效应。【方法】对小麦骨干亲本周8425B及其207份衍生品种开展叶锈病苗期抗性接种鉴定,同时利用叶锈病抗性基因LrZH84和LrZH22/Lr13的连锁标记或功能标记进行基因型检测。【结果】207份衍生品种对目前叶锈菌中强毒力优势生理小种KKSQ、TKTS、TKGQ均表现中抗及其以上水平的材料有42份,所占比例为20.3%;31份衍生品种仅携带LrZH84基因,其中对鉴定的3个生理小种表现中抗水平的分别有8、10、8份,所占比例分别为25.8%、32.3%、25.8%;46份衍生品种仅携带LrZH22/Lr13基因,其中对鉴定的3个生理小种表现中抗水平的分别有21、31、25份,所占比例分别为45.7%、67.4%、54.3%;14份衍生品种同时携带LrZH84和LrZH22/Lr13抗病基因,其中对鉴定的3个生理小种表现中抗水平的分别有9、11、11份,所占比例分别为64.2%、78.6%、78.6%;116份衍生品种均不携带LrZH84和LrZH22/Lr13抗病基因,其中对鉴定的3个生理小种表现中抗水平的分别有19、21、20份,所占比例分别为16.4%、18.1%、17.2%。【结论】叶锈病抗性基因LrZH22/Lr13的苗期抗性效应优于LrZH84,聚合两个叶锈病抗性基因的苗期抗性效应优于单个基因;需加强不同抗性基因的聚合,培育新的具有持久抗性的小麦新品种从而有效控制小麦叶锈病的流行。【Objective】Researches were conducted to find out the distribution and genetic effect of wheat leaf rust resistance gene LrZH84 and LrZH22/Lr13 in the founder parent Zhou 8425B and its 207 derived varieties.【Method】Leaf rust resistance identification was carried out on wheat backbone parent 8425B and its 207 derivative varieties at seedling stage,and the linkage or functional markers of leaf rust resistance genes LrZH84 and LrZH22/Lr13 were used for genotyping.【Result】Among the 207 derivative varieties,42 of them,accounting for 20.3%,showed moderate or high resistance to Puccinia triticina pathotypes KKSQ,TKTS,TKGQ,which were the dominant physiological race of virulence in China.31 of them only carry leaf rust resistance gene LrZH84,and 8,10 and 8 of them show moderate resistance to Puccinia triticina pathotypes KKSQ,TKTS and TKGQ,respectively,accounting for 25.8%,32.3%and 25.8%respectively;46 of them only carry leaf rust resistance gene LrZH22/Lr13,and 21,31 and 25 of them show moderate resistance to Puccinia triticina pathotypes KKSQ,TKTS and TKGQ,respectively,accounting for 45.7%,67.4%and 54.3%,respectively;14 of them carry both leaf rust resistance gene LrZH84 and LrZH22/Lr13,and 9,11 and 11 of them show moderate resistance to Puccinia triticina pathotypes KKSQ,TKTS and TKGQ,accounting for 64.2%,78.6%and 78.6%,respectively;the rest of 116 varieties do not carry any of its resistance genes,and there are 19,21 and 20 of them showing moderate resistance to Puccinia triticina pathotypes KKSQ,TKTS and TKGQ,accounting for 16.4%,18.1%and 17.2%,respectively.【Conclusion】At seedling stage,the resistance effect of LrZH22/Lr13 was better than that of LrZH84,and the aggregated resistance effect of the two genes was better than that of a single gene.It is necessary to strengthen the aggregation of different resistance genes and cultivate new wheat varieties with durable resistance to effectively control the epidemic of wheat leaf rust.

关 键 词：小麦叶锈病 周8425B 抗叶锈病基因 衍生系 遗传解析 

分 类 号：S512.1[农业科学—作物学]