玉米秸秆水提-甘油协同蒸汽爆破预处理及其丁醇发酵研究  

Water extraction coupled glycerol-assisted steam explosion pretreatment of corn stover and its butanol fermentation

作　　者：李晓娟 董红丽 张宏森 宋安东[1] 聂文喜 王风芹[1] LI Xiaojuan;DONG Hongli;ZHANG Hongsen;SONG Andong;NIE Wenxi;WANG Fengqin(College of Life sciences,Henan Agricultural University,Zhengzhou 450046,China;Henan Rural Industry Development Service Center,Zhengzhou 450002,China)

机构地区：[1]河南农业大学生命科学学院,河南郑州450046 [2]河南省乡村产业发展服务中心,河南郑州450002

出　　处：《河南农业大学学报》2023年第6期1035-1043,共9页Journal of Henan Agricultural University

基　　金：河南省科技攻关项目(222102520015);中原科技创新领军人才项目(204200510018)。

摘　　要：【目的】构建玉米秸秆水提-甘油协同蒸汽爆破预处理技术体系,利用预处理玉米秸秆非水洗水解液中的甘油和葡萄糖共发酵生产丁醇。【方法】分别对玉米秸秆进行蒸汽爆破、水浸润蒸汽爆破、甘油协同蒸汽爆破及水提-甘油协同蒸汽爆破,研究不同预处理对玉米秸秆残渣中三素含量(纤维素、半纤维素和木质素)及纤维素酶解效率和葡萄糖产量的影响;利用巴氏梭菌(Clostridium pasteurianum)CICC10391对含甘油玉米秸秆水解液进行厌氧瓶发酵生产丁醇,同时研究玉米浆对丁醇发酵的影响,并进行5 L发酵罐扩大发酵试验。【结果】经水提-甘油协同蒸汽爆破预处理后,玉米秸秆纤维素酶解效率达89.7%,葡萄糖产量为280.8 g·kg^(-1),较单独蒸汽爆破预处理分别提高68.9%和69.8%;巴氏梭菌CICC10391可同时利用水解液中的葡萄糖和甘油发酵生产丁醇,利用率分别为100.0%和78.9%,发酵50 h后,丁醇产量达到7.3 g·L^(-1)。【结论】实现了水提-甘油协同蒸汽爆破预处理玉米秸秆的高效酶解及含甘油水解液发酵生产丁醇,为利用玉米秸秆和生物柴油副产物甘油高值转化丁醇提供了技术支撑。【Objective】This research aims to establish water extraction coupled glycerol-assisted steam explosion pretreatment technology system for corn stover bio-transformation,and utilize the co-fermented of glycerol and glucose in the non-washed hydrolysate of pretreatment corn straw to produce bio-butanol.【Method】Corn stover was pretreated by 4 different pretreatments including steam explosion,watersoaked steam explosion,glycerol-assisted steam explosion,and water extraction coupled glycerol-assisted steam explosion,respectively.Components(cellulose,hemicellulose and lignin)content,cellulose enzymatic hydrolysis efficiency and glucose yield of pretreated corn stover were analyzed to evaluate the effectiveness of different pretreatments.Butanol fermentation with Clostridium pasteurinum CICC10391 from glycerol and glucose in the enzymatic hydrolysate of corn stover pretreated by water extraction coupled glycerol-assisted steam explosion was performed in anaerobic flask.The effect of corn slurry on butanol fermentation was also investigated.Finally,butanol expanded fermentation was carried out in 5 L fermenter.【Result】The cellulose enzymatic hydrolysis efficiency of corn stover pretreated with water extraction coupled glycerol-assisted steam explosion reached 89.7%,and the glucose yield was 280.8 g·kg^(-1) corn stover,which was 68.9%and 69.8%higher than that pretreated by steam explosion alone,respectively.Clostridium pasteurianum CICC10391 can simultaneously utilize glucose and glycerol in the hydrolysate to produce butanol,with a utilization rate of 100.0%and 78.9%,respectively.After 50 hours of fermentation,the butanol yield was 7.3 g·L^(-1).【Conclusion】This study achieved efficient enzymatic hydrolysis of corn stover with water extraction coupled glycerol-assisted steam explosion pretreatment and efficient production of butanol from hydrolysate,providing a technical support for the high value conversion of corn stover and biodiesel by-product glycerol into bio-butanol.

关 键 词：水提 甘油协同蒸汽爆破 玉米秸秆 水解液 丁醇发酵 

分 类 号：Q939.97[生物学—微生物学]