褪黑素通过H_(2)O_(2)调控盐胁迫下小豆Na^(+)/K^(+)平衡机制  

The Mechanism of Melatonin Regulating Na^(+)/K^(+) Balance by Mediating H_(2)O_(2) in Adzuki Bean under Salt Stress

作　　者：王彪 宋世佳[2] 李东晓[1] 董伟欣 张月辰 WANG Biao;SONG Shijia;LI Dongxiao;DONG Weixin;ZHANG Yuechen(College of Agronomy,Hebei Agricultural University,State Key Laboratory of North China Crop Improvement and Regulation,Key Laboratory of Crop Growth Regulation of Hebei Province,Baoding 071001,China;Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050031,China;Hebei Open University,Shijiazhuang 050080,China)

机构地区：[1]河北农业大学农学院,华北作物改良与调控国家重点实验室,河北省作物生长调控实验室,河北保定071001 [2]河北省农林科学院,河北石家庄050031 [3]河北开放大学,河北石家庄050080

出　　处：《华北农学报》2023年第6期62-71,共10页Acta Agriculturae Boreali-Sinica

基　　金：河北省自然科学基金(C2021204045,C2022204070);河北省杂粮杂豆产业技术创新团队“质量提升与品牌培育”(HBCT2023050204);财政部和农业农村部:国家现代农业产业技术体系-食用豆(CARS-08-G-22)。

摘　　要：为探究褪黑素对盐胁迫下小豆幼苗生长发育的影响,以保红876为材料,设置4个处理:CK(清水)、S(60 mmol/L NaCl)、MT(50μmol/L褪黑素)和S-MT(60 mmol/L NaCl和50μmol/L褪黑素),并分析了小豆幼苗生长指标、光合指标、矿质元素离子含量和抗氧化酶活性的变化。结果表明,在盐胁迫条件下,较CK处理,小豆幼苗的生长受到明显抑制,叶绿素含量、净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)和胞间CO_(2)浓度(Ci)显著降低;叶、茎中Na^(+)含量显著上升,叶片中Ca^(2+)含量显著上升而Mg^( 2+)含量则显著下降,豆苗Na^(+)/K^(+)比值显著增加;MDA和H_(2)O_(2)含量显著上升,破坏了膜脂选择透性。盐胁迫下施加褪黑素后,较S处理,显著提高了豆苗株高、叶面积和生物量,总根长显著增加;同时提高了叶绿素含量、叶片Pn、Tr和Ci;叶、茎中Na^(+)和K^(+)含量显著降低,叶片Mg^( 2+)含量显著升高同时Ca^(2+)含量显著降低,豆苗Na^(+)/K^(+)比值降低;MDA、O_(2)^(-)和H_(2)O_(2)含量下降;提高了叶片和根系SOD、POD和CAT活性。正常条件下,施加褪黑素可以促进豆苗的生长,提高幼苗的光合能力,提高了可溶性蛋白含量。对15个代表指标进行Spearman相关分析,发现总根长、叶绿素含量、净光合速率、抗氧化酶活性均与Na^(+)/K^(+)比值和ROS含量呈显著或极显著负相关。综上所述,盐胁迫下,褪黑素通过激活了豆苗抗氧化酶活性,可直接和间接的减少H_(2)O_(2)含量,保持膜脂渗透稳定性,从而利于植物体内Na^(+)/K^(+)平衡;同时,H_(2)O_(2)和Ca^(2+)作为信号分子,在褪黑素调节细胞内离子平衡的过程中存在协同效应,从而提高植物的耐盐能力。In order to explore the effects of melatonin on the growth and development of adzuki bean seedlings under salt stress,a phytotron experiment was conducted with Baohong 876 as the material and four treatments:CK(Clear water),S(60 mmol/L NaCl),MT(50μmol/L melatonin)and S-MT(60 mmol/L NaCl and 50μmol/L melatonin).The growth indexes,photosynthetic indexes,mineral ion contents and antioxidant enzyme activities of adzuki seedlings were analyzed using classical physicochemical determination.The results showed as follows:under salt stress,compared with CK treatment,the growth of adzuki bean seedlings was significantly inhibited,chlorophyll content,net photosynthetic rate(Pn),transpiration rate(Tr),stomatal conductivity(Gs)and intercellular CO_(2)concentration(Ci)were significantly decreased;the contents ofNa^(+)in leaves,stems were significantly increased.Meantime,the content of Ca^(2+)in leaves was significantly increased while the content of Mg^( 2+)was significantly decreased,and the ratio ofNa^(+)/K^(+)was significantly increased.The contents of MDA and H_(2)O_(2)increased significantly,which damaged the selective permeability of membrane lipid.Compared with S treatment,the plant height,leaf area,biomass,and total root length of adzuki bean seedlings were significantly increased under S-MT condition.Meantime,chlorophyll content,leaf Pn,Tr,and Ci were increased.The contents ofNa^(+)and K^(+)in leaves,stems were significantly decreased,the content of Mg^( 2+)in leaves was significantly increased and Ca^(2+)was significantly decreased,and theNa^(+)/K^(+)ratio was decreased.The contents of MDA,O_(2)^(-) and H_(2)O_(2)decreased.The levels of SOD,POD and CAT activity in leaves and roots were increased.Under normal conditions,the application of melatonin could promote the growth of adzuki bean seedlings,improve the photosynthetic capacity of seedlings,and increase the content of soluble protein.Spearman correlation analysis was performed on 15 representative indicators,and it was found that total root length,chlorophyll c

关 键 词：小豆 褪黑素 盐胁迫 Na^(+)/K^(+)平衡 

分 类 号：S521.01[农业科学—作物学]