陆地棉SKS基因家族全基因组特征分析与GhSKS13抗黄萎病功能解析  

Genome-wide Characterization of SKS Gene Family in Gossypium hirsutum and Functional Analysis of GhSKS13 in Plant Resistance to Verticillium Wilt

作　　者：陆承哲 贾培 武盼 唐叶 石琳芳 陈爱民 彭清忠[1] 吴家和[2] LU Chengzhe;JIA Pei;WU Pan;TANG Ye;SHI Linfang;CHEN Aimin;PENG Qingzhong;WU Jiahe(College of Biology and Environmental Sciences,Jishou University,Jishou 416000,China;Institute of Microbiology,Chinese Academy of Sciences,State Key Laboratory of Plant Genomics,Beijing 100101,China;Ministry of Agriculture and Rural Affairs Laboratory of Northwest Inland Cotton Bio-breeding and Integrated Utilization,Innovation Join Hope Seed Corporation,Ltd.,Changji 831100,China)

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000 [2]中国科学院微生物研究所,植物基因组学国家重点实验室,北京100101 [3]九圣禾种业股份有限公司,农业农村部西北内陆棉花生物育种与综合利用全国重点实验室,新疆昌吉831100

出　　处：《华北农学报》2023年第6期156-167,共12页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金(32272117);棉花生物育种与综合利用全国重点实验室(CB2023B01)。

摘　　要：鉴定陆地棉SKS基因家族成员,解析其演化规律,为棉花抗性育种提供新的候选基因。基于已公布的陆地棉遗传标准系TM-1基因组数据,以陆地棉品种中棉-14为试验材料。通过生物信息学对陆地棉SKS家族成员进行全基因组鉴定,并对其家族成员的染色体分布、进化关系、基因结构、共线性关系等进行预测分析。通过实时荧光定量聚合酶链式反应分析GhSKS13的表达模式,并利用病毒诱导的基因沉默技术初步探究陆地棉SKS13在棉花抵御大丽轮枝菌过程中的功能。共鉴定到48个陆地棉SKS基因,不均匀分布于陆地棉19条染色体上,聚类分析分为5个亚组,基因序列具有较高保守性,共线性关系分析显示,陆地棉SKS基因家族受到纯化选择。组织模式表达分析显示,GhSKS13在陆地棉根组织中优势表达,并且受大丽轮枝菌诱导显著上调表达。GhSKS13沉默植株对大丽轮枝菌抗性减弱,同时抑制病程相关基因GhPR1、GhPR2、GhPR3和GhPR5表达。大丽轮枝菌入侵GhSKS13沉默植株,其过氧化氢(H 2 O 2)沉积明显低于对照,暗示GhSKS13促进活性氧(ROS)的形成。总之,明确了陆地棉SKS家族成员的系统进化关系、染色体分布特征和基因结构特征;并阐明了GhSKS13参与棉花对大丽轮枝菌抗性反应。Genomic-wide identification of systematic work on the SKS gene family in cotton has not been reported.Identifying SKS(Skewed5 similar)gene family members and analyzing evolutionary relationship in Gossypium hirsutum,and analyzing the mechanism of GhSKS13 gene regulating cotton disease resistance are expected to provide a new direction for the cultivation of resistant plants.Here,the members of upland cotton SKS family were identified by bioinformatics at the genome-wide based on published genomic data of upland cotton genetic standard line TM-1,and G.hirsutum variety CRI-14 was used as the material.And chromosome distribution,evolutionary relationships,gene structure,and collinearity of SKS family members were predicted.Then,the expression pattern of GhSKS13 was analyzed by Real-time Quantitative PCR and virus-induced gene silencing was employed for preliminary investigation of the function of GhSKS13 in cotton resistance to Verticillium dahliae.A total of 48 upland cotton SKS genes were identified,unevenly distributed on 19 chromosomes,clustered into 5 subgroups with highly conserved gene sequences.Collinearity analysis revealed that the upland cotton SKS gene family was subject to purifying selection.Expression pattern analysis showed that GhSKS13 was predominantly expressed in upland cotton root tissues and significantly up-regulated by V.dahliae infection.GhSKS13-silenced reduced plants resistance to V.dahliae and suppressed the expression of the pathogen-related genes including GhPR1,GhPR2,GhPR3 and GhPR5 compared to control plants.GhSKS13-silenced plants invaded by V.dahliae showed significantly lower hydrogen peroxide(H 2O 2)deposition compared to the control,suggesting that GhSKS13 promotes the formation of reactive oxygen species(ROS).In conclusion,this study clarified the phylogenetic relationships,chromosomal distribution characteristics and gene structure characteristics of upland cotton SKS family members,and elucidated the involvement of GhSKS13 in upland cotton plant resistance to V.dahliae.

关 键 词：陆地棉 全基因组鉴定 大丽轮枝菌 SKS基因 活性氧 

分 类 号：S435.6[农业科学—农业昆虫与害虫防治]