不同NDF水平开食料对羔羊生长轴及十二指肠生长相关基因表达的影响  

Effect of Different NDF Levels of Starter on Growth Axis and Duodenum-Related Gene Expression in Lambs

作　　者：韩晓霞 赵海碧 刘国华[1] 吕凤 庞鑫 杨帆 汪晓娟[1] HAN Xiaoxia;ZHAO Haibi;LIU Guohua;L Feng;PANG Xin;YANG Fan;WANG Xiaojuan(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070

出　　处：《华北农学报》2023年第6期211-217,共7页Acta Agriculturae Boreali-Sinica

基　　金：甘肃省自然科学基金项目(20JR10RA538);国家自然科学基金项目(31760682)。

摘　　要：旨在研究不同NDF水平开食料对羔羊生长轴及十二指肠生长相关基因表达的影响,以期为羔羊开食料适宜NDF水平提供参考。选用36只湖羊公羔,随机分为15%和25%NDF水平组。56日龄时,每组选择6只羔羊屠宰,称量组织器官质量,并测定相关基因的表达量。结果表明:不同NDF水平开食料对组织器官的质量和指数均无显著影响(P>0.05);NDF水平15%组羔羊下丘脑生长激素释放激素(GHRH)、肝脏胰岛素样生长因子-1(IGF-1)、十二指肠IGF-1和十二指肠胰岛素样生长因子1受体(IGF-1R)表达量均显著高于25%组(P<0.05);NDF水平25%组垂体生长激素(GH)和十二指肠生长激素受体(GHR)表达量极显著高于15%组(P<0.01);羔羊平均日增质量与下丘脑GHRH表达量极显著负相关(P<0.01)、与肝脏GHR、十二指肠IGF-1表达量显著负相关(P<0.05),与垂体GH、十二指肠GHR表达量显著正相关(P<0.05);垂体GH与肝脏GHR、IGF-1、十二指肠IGF-1、IGF-1R表达量显著或极显著负相关(P<0.05或P<0.01),与十二指肠GHR表达量极显著正相关(P<0.01);肝脏GHR与肝脏IGF-1、十二指肠IGF-1表达量极显著正相关(P<0.01),与十二指肠GHR表达量极显著负相关(P<0.01)。综上所述,与15%水平组相比,饲喂25%NDF水平开食料更能提高羔羊生长轴GH-GHR水平相关基因的表达。The aim of this trial was to investigate the effect of different levels of NDF on the expression of genes related to growth axis and duodenal growth in lambs,in order to provide a reference for the appropriate NDF level for lamb starter.Thirty-six male Hu lambs were selected for the trial and randomly divided into 15%and 25%NDF level groups.At 56 days of age,six lambs from each group were selected for slaughter,weighed for tissue and organ,and the expression of relevant genes was measured.The results showed that there was no significant effect(P>0.05)of NDF levels in the starter diet on tissue and organ mass and index;in the 15%group of lambs,hypothalamic growth hormone releasing hormone(GHRH),hepatic insulin-like growth factor-1(IGF-1),duodenal IGF-1 and IGF-1 receptor(IGF-1R)expressions were significantly higher in the 25%group(P<0.05);the expression of pituitary growth hormone(GH)and growth hormone receptor(GHR)in the duodenum were significantly higher in the 25%group than in the 15%group(P<0.01).The average daily gain of lambs was strongly negatively correlated with hypothalamic GHRH expression(P<0.01),significantly negatively correlated with hepatic GHR and duodenal IGF-1 expression(P<0.05),and significantly positively correlated with pituitary GH and duodenal GHR expression(P<0.05);pituitary GH was significantly or highly significantly negatively correlated with hepatic GHR,IGF-1,duodenal IGF-1 and IGF-1R expression(P<0.05 or P<0.01),and strongly significantly positively correlated with duodenal GHR expression(P<0.01);hepatic GHR was strongly significantly positively correlated with hepatic IGF-1,duodenal IGF-1 expression(P<0.01),and strongly significantly negatively correlated with duodenal GHR expression(P<0.01).In conclusion,the expression of genes related to GH-GHR levels in the growth axis of lambs is enhanced by feeding 25%NDF level open feed compared to the 15%level group.

关 键 词：羔羊 开食料 生长轴 十二指肠 基因表达 

分 类 号：S811[农业科学—畜牧学] S815.2[农业科学—畜牧兽医]