条斑紫菜绿斑病优势病原菌的鉴定及其生长特性与药敏分析  

Identification,Growth Characteristics and Drug Sensitivity Analysis of Dominant Pathogen of Green Spot Disease of Porphyra yezoensis

作　　者：王颖 赵龙成 王浩同 杜建周 王洪斌 赵晓恒 程汉良 许建和 陈香凝 丁祝进 WANG Ying;ZHAO Longcheng;WANG Haotong;DU Jianzhou;WANG Hongbin;ZHAO Xiaoheng;CHENG Hanliang;XU Jianhe;CHEN Xiangning;DING Zhujin(School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Key Laboratory of Marine Biotechnology,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Key Laboratory of Marine Bioresources and Environment,Jiangsu Ocean University,Lianyungang 222005,China;Co-innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Ocean University,Lianyungang 222005,China;School of Materials Science and Engineering,Yancheng Institute of Technology,Yancheng 224051,China)

机构地区：[1]江苏海洋大学海洋科学与水产学院,江苏连云港222005 [2]江苏海洋大学江苏省海洋生物技术重点实验室,江苏连云港222005 [3]江苏海洋大学江苏省海洋生物资源与环境重点实验室,江苏连云港222005 [4]江苏海洋大学江苏省海洋生物产业技术协同创新中心,江苏连云港222005 [5]盐城工学院材料科学与工程学院,江苏盐城224051

出　　处：《江苏海洋大学学报（自然科学版）》2023年第4期6-12,共7页Journal of Jiangsu Ocean University:Natural Science Edition

基　　金：江苏省农业科技自主创新资金项目(CX(20)3110);江苏现代农业(紫菜)产业技术体系连云港推广示范基地项目(JATS[2022]172);江苏省大学生创新创业训练项目(SY202211641631002);江苏高校优势学科建设工程(海洋科学)项目。

摘　　要：从条斑紫菜绿斑病样品中分离获得优势致病菌,通过16S rRNA测序进行菌种鉴定,建立快速检测的LAMP技术,并进行生长特性及药敏实验分析。使用16S rRNA测序鉴定表明,该致病菌为深海微小杆菌(Microbacter bathyae)。根据OD值建立不同温度和培养基中的生长曲线,分析深海微小杆菌的生长特性。利用K-B纸片法进行药敏试验,分析其对20种抗生素的药物敏感性。结果表明,条斑紫菜绿斑病的优势病原菌为深海微小杆菌,对其生长特性与药物敏感性进行分析,可为条斑紫菜绿斑病病原的防治提供参考依据。In this study,the dominant pathogenic bacteria were isolated from the samples of Porphyra yezoensis,and identified by 16S rRNA sequencing.A rapid detection LAMP technique was established,and growth characteristics and drug sensitivity experiments were analyzed.First of all,16S rRNA sequencing identification showed that the pathogenic bacterium was Microbacter bathyae.According to OD value,the growth curves in different temperatures and medium were established to analyze the growth characteristics of Microbacter bathyae.Then,the drug sensitivity test was carried out by K-B disk method to analyze its drug sensitivity to 20 kinds of antibiotics.In this study,the dominant pathogen of porphyra green spot was identified as Microbacter bathyae,and its growth characteristics and drug sensitivity were analyzed,which provided a reference for the prevention and treatment of Porphyra yezoensis.

关 键 词：紫菜绿斑病 优势病原菌 深海微小杆菌 生长曲线 药敏试验 

分 类 号：S946.2[农业科学—水产养殖]