玉竹蔗糖合成酶(SUS)基因家族的鉴定、表达分析及PoSUS1基因的克隆  

Identification and Expression Profiling of the Sucrosesynthase (SUS) Gene Family and Cloning of PoSUS1 in Polygonatum odoratum

作　　者：潘根 秦裕辉[1,2] 张水寒 PAN Gen;QIN Yuhui;ZHANG Shuihan(Institute of Chinese Materia Medica,Hunan Academy of Chinese Medicine,Changsha,Hunan 410013,China;Colleges of Chi-nese Medicine,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,Hunan 410205,China)

机构地区：[1]湖南省中医药研究院中药资源研究所,湖南长沙410013 [2]湖南中医药大学中医学院,湖南长沙410208 [3]中国农业科学院麻类研究所,湖南长沙410205

出　　处：《热带作物学报》2023年第12期2469-2476,共8页Chinese Journal of Tropical Crops

基　　金：湖南省科技成果转化及产业化计划项目(No.2020SK2029);湖南省中医药科研计划项目(No.A2022002,No.A2022005)。

摘　　要：多糖是玉竹的质量标志物,在免疫调节、抗肿瘤等方面具有显著的药理作用。作为多糖合成的关键酶之一,蔗糖合成酶(sucrose synthase,SUS)一直是揭示植物多糖合成的重要研究内容。基于转录组序列信息,本研究利用生物信息学手段对玉竹SUS基因家族及其成员进行鉴定,并利用荧光定量PCR(qPCR)对其成员表达模式进行分析。结果表明:玉竹转录组共获得8个具有ORF序列的PoSUS基因家族成员,其编码蛋白质含有111~310个氨基酸,分子量为12.81~35.43 kDa,其理论等电点为5.83~9.18。系统进化树分析表明,8个PoSUS基因家族成员可分为3个亚家族,其中第Ⅲ亚家族基因成员数目最多。亚细胞定位预测显示大多数PoSUS蛋白定位在叶绿体。基因表达模式分析表明,PoSUS1和PoSUS6基因在多糖积累的根茎组织中表达量最高,且高多糖种质HN1中PoSUS1和PoSUS2表达量显著高于低多糖种质AH2。此外,本研究还从种质HN1和AH2克隆得到PoSUS1基因CDS序列,其编码蛋白在2份种质间存在3处氨基酸差异,且这些差异位于PoSUS1蔗糖合成酶结构域。本研究结果为深入研究玉竹SUS基因功能奠定基础,也为玉竹药用品质形成分子机制研究提供理论依据。Polysaccharide is a quality marker of Polygonatum odoratum,which has significant pharmacological effects in immune regulation and anti-tumor.As one of the key enzymes in polysaccharide synthesis,sucrosesynthase(SUS)has always been an important research field to reveal the synthesis of plant polysaccharides.Based on transcriptom data of Polygonatum odoratum,the SUS gene family members were identified by bioinformatics,and their expression pro-filing were analyzed using qPCR.The results showed that eight PoSUS gene were identified,their protein ranged from 111 to 310 amino acid residues(aa)in length,and relative molecular weight varied from 12.81 kDa to 35.43 kDa,isoelectric point(pI)in the range of 5.83 to 9.18;phylogenetic analysis indicated that eight PoSUS genes were divided into 3 subfamily,and the subfamilyⅢincluded the largest PoSUS genes family member;subcellural localization analysis showed that most of PoSUS proteins were located in chloroplast.Additionally,expression patterns analysis revealed that PoSUS1 and PoSUS6 genes were preferably expressed in rhizome,and the transcript levels of PoSUS1 and PoSUS2 were higher in high-polysaccharide cultivar HN1 than those in a low-polysaccharide cultivar AH2.In addition,the CDS of PoSUS1 gene were cloned from HN1 and AH2,there are three amino acid difference between HN1 and AH2,which located in sucrose synthase domain.Our findings can laid a basis for the further study of functional analysis of SUS genes,and provided a theoretical basis for analyzing molecular mechanism of the formation mechanism of me-dicinal quality of Polygonatum odoratum.

关 键 词：玉竹 SUS基因家族 表达分析 PoSUS1 

分 类 号：S567.239[农业科学—中草药栽培]