利用全因子实验设计模型优化角蛋白载药pH响应型纤维的释药性能  

Electrospun nanofibers with pH-responsive operational factors for controlling of chemotherapeutic drugs release performance

作　　者：罗璐 张晶 雷悦 LUO Lu;ZHANG Jing;LEI Yue(School of Textile Science and Engineering,Xi’an Polytechnic University,Xi’an 710048,China)

机构地区：[1]西安工程大学纺织科学与工程学院,陕西西安710048

出　　处：《实验技术与管理》2023年第11期21-27,共7页Experimental Technology and Management

基　　金：西安工程大学博士科研启动基金(20141201);大学生创新创业项目项目(202210709035)。

摘　　要：为研究纤维基载药羊毛角蛋白支架的肠道抗癌释药性能,该文选择5-氟尿嘧啶(5-FU)作为药物模型,使用全因子矩阵实验设计,在不同酸碱环境下,通过在两水平上优化多个显著性因子的参数,提高pH敏感性释放性能。这种全因子实验设计方法既可用于确定复合生物功能系统的主要效应,也可用于研究多因素相互作用的影响。扫描电子显微镜(SEM)图像显示,在15 kV静电纺丝电压下,可得到具有均匀结构的复合纳米纤维基质;同时,傅里叶变换红外光谱(FT-IR)证实羊毛角蛋白在1630和1550 cm^(-1)处有特征峰。使用紫外-可见分光光度计(UV-Vis)检测药物缓释性能,测试结果表明:羊毛角蛋白与聚L-丙交酯(Poly-L-lactide,PLLA)的比例是影响药物缓释性能的主要因素。通过全因子矩阵实验设计优化后,在120 h内,含有50%羊毛角蛋白的复合载药纳米纤维在pH值为7.4的环境中释放了48.5%的药物,而在pH值为6.0的环境中释放了83.7%的药物。证明通过优化调整操作因子,实现了羊毛角蛋白纤维支架的pH响应性显著提高。In order to investigate the drug release performance of the fiber-based drug-loaded wool keratin scaffold for intestinal anti-cancer applications,this study selected 5-fluorouracil(5-FU)as the drug model and employed a full factorial design of experiment to optimize the pH sensitivity release performance in different acidic and alkaline environments with multiple significant factors at two levels.This full-factorial design of experimental method can be applied both in identifying the main effects of this composite biofunctional system and in investigating the effects of multiple-factor interactions.The scanning electron microscopy(SEM)images indicated that with lower electrospun voltage at 15 kV,a more homogenous microstructure of the composite nanofibers can be observed.The Fourier transform infrared spectroscopy(FT-IR)test detected the characteristic peaks of keratin at 1630 and 1550 cm^(−1).The releasing performances are examined by ultraviolet and visible spectrophotometry(UV-Vis).Results illustrated that the ratio between keratin and Poly-L-lactide(PLLA)is a major factor in controlling the sustained-releasing performance.After optimization through full factor experimental design,the composite drug-loaded nanofibers with 50%,keratin,48.5%of 5-FU is released in pH 7.4 compared to 83.7%of 5-FU is released in pH 6.0 in 120 h.By optimizing and adjusting the operational factors,the pH responsiveness of the wool keratin fiber scaffold is significantly improved.

关 键 词：静电纺丝 纳米纤维 羊毛角蛋白 药物缓释 

分 类 号：TS195.6[轻工技术与工程—纺织化学与染整工程]