星康吉鳗精子的超微结构研究  

A study on sperm ultrastructure in the whitespotted conger,Conger myriaster

作　　者：史宝[1] 王成刚 汤晓华 赵新宇 晏科文 马晓东 SHI Bao;WANG Cheng-gang;TANG Xiao-hua;ZHAO Xin-yu;YAN Ke-wen;MA Xiao-dong(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture and Rural Affairs,Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266071,China;Haiyang Yellow Sea Aquatic Product Co.,Ltd.,Yantai 265100,China)

机构地区：[1]中国水产科学研究院黄海水产研究所、农业农村部海洋渔业可持续发展重点实验室、青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室,山东青岛266071 [2]海阳市黄海水产有限公司,山东烟台265100

出　　处：《海洋科学》2023年第10期112-120,共9页Marine Sciences

基　　金：中国水产科学研究院黄海水产研究所基本科研业务费资助项目(20603022021004,20603022023023);山东省重点研发计划项目(2021LZGC028);国家重点研发计划项目(2019YFD0900503);国家现代农业产业技术体系资助项目(CARS-47)。

摘　　要：为探讨星康吉鳗(Conger myriaster)精子的超微结构和形态,应用扫描电镜和透射电镜对星康吉鳗精子结构进行观察。结果表明,精子由头部、中段和鞭毛3部分组成,有其独特的结构,总长度为35.75±1.15μm。精子头部为新月形,主要由细胞核构成,细胞核内有核泡,无顶体结构。精子头部的质膜内包含单一的线粒体。精子头部长为3.33±0.16 um,头宽为1.12±0.13 um。在精子头部的凸面上,有4条从中段到头端的条纹。精子中段伸出一支根,支根位于精子的中段末端。精子中段长度为0.55±0.05 um,支根长度为1.38±0.08 um、直径为90.48±6.06 nm。精子尾部鞭毛细长,鞭毛横切面呈圆形,无侧鳍,鞭毛的轴丝结构为“9+0”型;一些鞭毛的末端呈现卷曲状,发育机制尚不明确。精子鞭毛长为31.16±1.51μm,鞭毛直径为0.17±0.01μm。通过比较分析发现精子的这些形态学特征不仅表现在星康吉鳗精子,还表现在鳗鲡目其他属的精子;表明是鳗鲡目精子的共同特征。本研究揭示了星康吉鳗精子的形态结构,为突破星康吉鳗人工繁殖技术提供了理论参考。Whitespotted conger,Conger myriaster,is one of the most valuable fishery resources in the seas around China,Korea,and Japan.Thus,it has become important to protect this species,as the annual commercial catch is decreasing.Despite being an important resource for East Asian countries,little is known about the reproductive biology of C.myriaster.Therefore,only very limited information is available regarding the reproductive characteristics of male C.myriaster.Teleost sperm is very morphologically diverse,particularly the sperm head shape;the shape,location,and number of mitochondria,and the structure and length of the flagellum.This study investigated the ultrastructure and morphology of C.myriaster sperm by scanning electron microscopy(SEM)and transmission electron microscopy(TEM).The SEM and TEM images revealed that the sperm were composed of a head,a middle piece,and a flagellum.C.myriaster sperm had a unique structure except for the common sperm characteristics of teleost.The total mean length was 35.75±1.15μm.The head of the spermatozoon was electron-dense and contained chromatin material forming the nucleus.The nucleus was asymmetrically shaped along the longitudinal axis.We observed nuclear vacuoles in the nucleus,which were not fixed in size or position.The head had no acrosomal structure.A single spherical mitochondrion was located along the longitudinal center line of the nucleus but was offset slightly to one side.The mitochondrion was surrounded by an outer mitochondrial membrane.The inner mitochondrial membrane extended inward,forming rich cristae.Ribonucleoprotein and matrix were scattered in the mitochondrion.The mean head length was 3.33±0.16μm,and the mean head width was 1.12±0.13μm.Four striae ran from the caudal portion to the cephalad on the convex surface of the head.Additionally,four striae arose from the proximal centriole.The middle piece was constricted and projected a short rootlet located at the end of the middle piece.The mean middle piece length was 0.55±0.05μm.The mean length of

关 键 词：星康吉鳗(Conger myriaster) 精子 超微结构 精子形态 

分 类 号：S962[农业科学—水产养殖]