川陈皮素调节CCL2-CCR2信号轴对牙周炎大鼠炎性损伤的影响  

The Impact of Nobiletin on the Inflammatory Injury in Rats with Periodontitis by Regulating the CCL2-CCR2 Signaling Axis

作　　者：喻锦娴[1] 俞梅[1] 李娜[1] YU Jinxian;YU Mei;LI Na(Wuhan Hospital of Traditional Chinese Medicine,Hubei Wuhan 430000,China)

机构地区：[1]湖北省武汉市中医医院口腔科,湖北武汉430000

出　　处：《河北医学》2023年第12期1937-1941,共5页Hebei Medicine

基　　金：湖北省武汉市中医药科研项目(编号:WZ22Z21)。

摘　　要：目的:通过构建牙周炎大鼠模型探讨川陈皮素对牙周炎大鼠炎性损伤的影响及作用机制。方法:采用丝线结扎联合10%蔗糖饮水建立牙周炎大鼠模型;将牙周炎大鼠分为模型组、川陈皮素低剂量组、川陈皮素中剂量组、川陈皮素高剂量组,每组10只,另取10只大鼠为对照组;川陈皮素低、中、高剂量组分别灌胃25mg/kg、50mg/kg、100mg/kg川陈皮素,对照组、模型组灌胃等量生理盐水。HE染色检测各组大鼠牙周组织病理形态变化;qPCR检测各组大鼠牙周组织C-C趋化因子配体2(CCL2)、C-C趋化因子受体2(CCR2)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、IL-6 mRNA水平;免疫组织化学染色检测各组大鼠牙周组织CCL2表达;Western blot检测各组大鼠牙周组织CCL2、CCR2、TNF-α、IL-1β、IL-6蛋白表达。结果:牙周炎大鼠模型建立4周后,与对照组相比,造模大鼠出现牙槽骨吸收、牙根松动有出血现象,表明牙周炎大鼠模型构建成功;与对照组相比,模型组大鼠牙周组织出现牙槽骨吸收、有大量炎性细胞浸润、CCL2阳性细胞数目增多,牙周组织CCL2、CCR2、TNF-α、IL-1β、IL-6 mRNA和蛋白水平均显著升高(P<0.05);与模型组相比,川陈皮素低、中、高剂量组大鼠牙周组织炎性细胞浸润逐渐减轻,成纤维细胞数量逐渐增加,CCL2阳性细胞数目减少,牙周组织CCL2、CCR2、TNF-α、IL-1β、IL-6水平显著降低,呈剂量依赖性(P<0.05)。结论:川陈皮素可能通过抑制CCL2-CCR2信号轴减轻牙周炎大鼠炎性损伤。Objective:To investigate the effects of Nobiletin on inflammatory injury in periodontal disease rats and its mechanism by constructing a model of periodontitis rats.Methods:The rat model of periodontal disease was established by silk thread ligation and 10%sucrose drinking water;periodontal disease rats were separated into a model group,a low-dose Nobiletin group,a medium dose Nobiletin group,and a highdose Nobiletin group,with 10 rats in each group.Another 10 rats were selected as the control group.The low,medium and high dose Nobiletin groups were administrated with 25mg/kg,50mg/kg and 100 mg/kg,respectively,while the control group and model group were orally administrated with the same amount of normal saline.HE staining was performed to detect pathological changes in the periodontal tissue of rats in each group;qPCR was applied to measure the levels of C-C chemokine ligand 2(CCL2),C-C chemokine receptor 2(CCR2),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and IL-6 mRNA in periodontal tissues of rats in each group.Immunohistochemical staining was applied to detect the expression of CCL2 in the periodontal tissue of rats in each group;Western blot was applied to detect the expression of CCL2,CCR2,TNF-α,IL-1β,and IL-6 proteins in the periodontal tissue of rats in each group.Results:Four weeks after the establishment of the periodontal disease rat model,compared with the control group,the model rats showed alveolar bone resorption,tooth root loosening and bleeding,indicated that the establishment of the periodontal disease rat model was successful;compared with the control group,the periodontal tissue of rats in the model group showed alveolar bone resorption,with a large number of inflammatory cell infiltration,the number of CCL2 positive cells increased,the mRNA and protein levels of CCL2,CCR2,TNF-α,IL-1β,IL-6 in periodontal tissue were obviously increased(P<0.05);compared with the model group,the inflamma-tory cell infiltration in the periodontal tissue of rats in the low,middle and high dose N

关 键 词：川陈皮素 C-C趋化因子配体2 C-C趋化因子受体2 牙周炎大鼠 

分 类 号：R285.5[医药卫生—中药学]