基于5-HT探讨电针刺激天枢、足三里对IBS-D大鼠的影响  

Effects of electroacupuncture stimulation of"Tianshu"(ST25)and"Zusanli"(ST36)on IBS-D rats based on 5-HT

作　　者：张之涵 刘羽彤 顾玲[1] 赵旭宇 花炀 葛飞[2] 陈美娟[1] ZHANG Zhihan;LIU Yutong;GU Ling;ZHAO Xuyu;HUA Yang;GE Fei;CHEN Meijuan(School of Medicine,Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210023,China;Hai'an Hospital of Chinese Medicine affiliated to Nanjing University of Chinese Medicine,Hai'an,Jiangsu 226600,China)

机构地区：[1]南京中医药大学医学院,江苏南京210023 [2]南京中医药大学附属海安市中医院,江苏海安226600

出　　处：《湖南中医药大学学报》2023年第12期2264-2271,共8页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金项目(81503374);江苏省自然科学基金项目(BK20181235);江苏省创新训练项目(202210315047Y)。

摘　　要：目的观察电针刺激天枢、足三里对腹泻型肠易激综合征(diarrheal irritable bowel syndrome,IBS-D)模型大鼠血清5-羟色胺(5-hydroxytryptamine,5-HT)含量及对结肠5-羟色胺受体3A型(5-hydroxytryptamine 3A receptor,5-HT3AR)蛋白表达量的影响,探讨“同经异腑合募配穴”指导下,电针刺激天枢、足三里治疗IBS-D大鼠的作用机制,为电针的临床应用提供实验依据。方法将24只SD雄性大鼠随机分为空白组、模型组、阿洛司琼组及电针组,每组6只。除空白组外,其余3组大鼠采用慢性束缚、避水应激及灌服番泻叶煎剂相结合的方法建立IBS-D大鼠模型。造模成功后,对电针组予以电针刺激双侧天枢、足三里,15 min/次;空白组、模型组和电针组均给予1 mL/100 g体质量生理盐水灌胃;阿洛司琼组给予1 mg/mL浓度的阿洛司琼溶液按1 mL/100 g体质量灌胃;4组均治疗1次/d,连续治疗14 d。观察大鼠一般情况;通过腹壁回撤反射(abdominal wall retraction reflex,AWR)实验进行内脏敏感性评估;记录大鼠体质量增长率的变化;计算大鼠粪便含水量;HE染色观察结肠组织形态;ELISA法检测血浆中的5-HT含量;Western blot检测目的蛋白5-HT3AR相对表达量。结果(1)大鼠一般情况:与模型组比较,电针组大鼠精神良好,活动正常,大便干湿适中,为成形粪粒。(2)体质量增长率:与治疗前比较,治疗后空白组、模型组、阿洛司琼组及电针组体质量增长率显著降低(P<0.001);与空白组比较,模型组与阿洛司琼组体质量增长率降低(P<0.01,P<0.05);与模型组、阿洛司琼组比较,电针组体质量增长率升高(P<0.05)。(3)粪便含水量:与治疗前比较,电针组粪便含水量显著降低(P<0.01);与空白组比较,模型组、阿洛司琼组粪便含水量高(P<0.001,P<0.05);与模型组、阿洛司琼组比较,电针组的粪便含水量降低(P<0.01,P<0.05)。(4)肠道容量阈值:与空白组比较,模型组肠道容量阈值显著降低(P<0.001);与模�Objective To observe the effects of electroacupuncture at"Tianshu"(ST25)and"Zusanli"(ST36)on 5-hydroxytryptamine(5-HT)content in serum and 5-hydroxytryptamine 3A receptor(5-HT3AR)protein expression in colon of model rats with diarrheal irritable bowel syndrome(IBS-D),and to explore the mechanism of action of treating IBS-D rats by electroacupuncture stimulation of"Tianshu"(ST25)and"Zusanli"(ST36)under the guidance of"combining he and mu points from the same type of meridians of different fu-organs",thus providing experimental evidence for the clinical application of electroacupuncture.Methods Twenty-four SD male rats were randomized into blank group,model group,alosetron group,and electroacupuncture group,with six rats in each group.Except the blank group,the other three groups of rats were used to establish IBS-D rat models by the combination of chronic restraint,water avoidance stress,and oral administration of Fanxieye(Sennae Folium)decoction.After successful modeling,the electroacupuncture group was given electroacupuncture at bilateral“Tianshu”(ST25)and"Zusanli"(ST36),15 min each time.The blank group,model group,and electroacupuncture group were administered 1 mL/100 g normal saline by gavage according to the body mass of rats,while the alosetron group was given 1 mg/mL concentration of alosetron solution by gavage at a dose of 1 mL/100 g based on body mass.All the four groups were treated once a day for 14 consecutive days.The general condition of rats was observed.Visceral sensitivity was evaluated by abdominal wall retraction reflex(AWR)experiment.In addition,the changes of body mass growth rate were recorded and the fecal water content was calculated.Besides,the morphology of colonic tissues was observed by HE staining,the 5-HT content in plasma was determined by ELISA,and the relative expression of target protein 5-HT3AR was measured by Western blot.Results(1)General condition of rats:Compared with the model group,the rats in electroacupuncture group were in good spirit with normal activity,and the

关 键 词：腹泻型肠易激综合征 同经异腑合募配穴 内脏高敏感性 5-HT 5-HT3AR 

分 类 号：R245.9[医药卫生—针灸推拿学]