重度子痫前期孕妇中NGF、Syncytin-1的表达及临床意义  

Expression and Clinical Significance of NGF and Syncytin-1 in Pregnant Women with Severe Preeclampsia

作　　者：屈艳艳 韩秋峪 孙亚薇 董苏薇 QU Yanyan;HAN Qiuyu;SUN Yawei(Xuzhou Medical University,Jiangsu 221004,China)

机构地区：[1]徐州医科大学,221004 [2]徐州医科大学附属医院妇产科,221006

出　　处：《医学研究杂志》2023年第11期137-141,共5页Journal of Medical Research

基　　金：江苏省徐州市重点研发计划(社会发展)项目(KC20087)。

摘　　要：目的研究重度子痫前期(severe preeclampsia,SPE)孕妇血清及胎盘组织中神经生长因子(nerve growth factor,NGF)、合胞素1(Syncytin-1)的表达水平及其临床意义。方法选取2021年12月~2022年6月于徐州医科大学附属医院产科住院行剖宫产分娩的孕妇90例,分为早发型SPE 30例(早发组)、晚发型SPE 30例(晚发组)及同期正常孕妇30例(对照组)。收集3组孕妇母血和胎盘组织,采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)和免疫组化(immunohistochemistry,IHC)染色检测各组NGF、Syncytin-1的表达水平,分析其临床意义。结果ELISA检测结果显示,与健康妊娠晚期孕妇比较,患有SPE的孕妇血清中NGF、Syncytin-1表达水平降低,且早发组低于晚发组(P<0.05)。IHC染色检测结果显示,与健康妊娠晚期孕妇比较,患有SPE的孕妇胎盘组织中NGF、Syncytin-1表达水平降低,且早发组低于晚发组(P<0.05)。结论3组孕妇的血清及胎盘组织中均有NGF、Syncytin-1的表达,且在正常孕妇、晚发型SPE、早发型SPE中的表达依次降低,可能是通过影响胎盘的正常发育参与SPE的发生、发展。Objective To study the expression levels and clinical significance of nerve growth factor(NGF)and Syncytin-1 in serum and placental tissues of pregnant women with severe preeclampsia(SPE).Methods A total of 90 pregnant women who underwent cesarean delivery in the Department of Obstetrics,the Affiliated Hospital of Xuzhou Medical University in December 2021 to June 2022 were selected,and they were divided into 30 cases of early-onset SPE(early-onset group),30 cases of late-onset SPE(late-onset group),and 30 cases of normal women in the same period(control group).The maternal blood and placental tissues of the three groups were collected,and the expression levels of NGF and Syncytin-1 in each group were detected by enzyme-linked immunosorbent assay(ELISA)and immunohistochemical(IHC)staining,and their clinical significance was analyzed.Results The results of the ELISA showed that compared with healthy pregnant women in the third trimester,the expression of NGF and Syncytin-1 in the serum of pregnant women with SPE was reduced,and the early-onset group was lower than those in the late-onset group(P<0.05).The results of IHC staining showed that compared with healthy pregnant women in the third trimester,the expression levels of NGF and Syncytin-1 in placental tissues were reduced in pregnant women with SPE,early-onset group was lower than those in the late-onset group(P<0.05).Conclusion The expression of NGF and Syncytin-1 in serum and placental tissues of the three groups of pregnant women was successively reduced,and the expression was successively reduced in normal pregnant women,late-onset SPE and early-onset SPE,which may be involved in the occurrence and development of SPE by affecting the normal development of the placenta.

关 键 词：重度子痫前期 神经生长因子 合胞素1 早发型重度子痫前期 晚发型重度子痫前期 

分 类 号：R71[医药卫生—妇产科学]