超快速液相色谱法同时测定刺五加中刺五加苷B和刺五加苷E的含量  

Simultaneous Determination of Eleutheroside B and Eleutheroside E in Acanthopanacis Senticosi Radix Et Rhizoma Seu Caulis by Ultra-fast Liquid Chromatography

作　　者：关皎 丁思宇 王宜优 葛胜宇 王军民[2] 朱鹤云 韩丽琴 GUAN Jiao;DING Siyu;WANG Yiyou;GE Shengyu;WANG Junmin;ZHU Heyun;HAN Liqin(School of Pharmacy,Jilin Medical University,Jilin 132013 Heilongjiang,China;Affiliated Hospital,Jilin Medical University,Jilin 132011 Heilongjiang,China)

机构地区：[1]吉林医药学院药学院,吉林吉林132013 [2]吉林医药学院附属医院,吉林吉林132011

出　　处：《中药新药与临床药理》2023年第11期1608-1611,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：吉林省教育厅“十三五”科学技术项目(JJKH20200466KJ);吉林省大学生创新创业项目(202113706074)。

摘　　要：目的建立一种同时测定刺五加中刺五加苷B和刺五加苷E含量的超快速液相色谱法。方法采用Shim-Pack XR-ODS柱(75 mm×3.0 mm,2.2μm);H_(2)O(A)-CH3OH(B)为流动相,梯度洗脱(0~1 min,10%B→35%B;1~5 min,35%B→50%B;5~8 min,50%B→60%B);流速:0.4 mL·min^(-1);柱温:30°C;进样量:5μL;平衡时间:5 min;检测波长:215 nm。结果刺五加苷B和刺五加苷E分别在10~400μg·mL^(-1)(r=0.9990)和5~200μg·mL^(-1)(r=0.9991)质量浓度范围内与峰面积呈良好的线性关系;平均回收率(n=9)分别为98.9%和98.2%;6批样品中刺五加苷B与刺五加苷E的含量范围分别为0.130%~0.145%和0.0708%~0.0776%。结论与《中国药典》方法比较,该方法稳定性和专属性好,分析时间短,可用于刺五加药材的质量控制研究。Objective An ultra-fast liquid chromatography(UFLC)method was established for the simultaneous determination of two active components(eleutheroside B and eleutheroside E)in Acanthopanacis Senticosi Radix et Rhizoma seu Caulis.Methods Shim-Pack XR-ODS column(75 mm×3.0 mm,2.2μm)was used for the separation of two analytes,water(A)-methanol(B)were used as the mobile phase,and gradient elution program(0-1 minutes,10%B→35%B;1-5 minutes,35%B→50%B;5-8 minutes,50%B→60%B)was adopted in this study.The equilibrium time was 5 minutes,and the flow rate was 0.4 mL·min^(-1).The detection wavelength was set at 215 nm and the column temperature was maintained at 30℃.The injection volume was 5μL.Results Eleutheroside B and eleutheroside E showed good linear relationship in the range of 10-400μg·mL^(-1)(r=0.9990)and 5-200μg·mL^(-1)(r=0.9991),respectively.The average recoveries(n=9)were 98.9%and 98.2%.The content ranges of eleutheroside B and eleutheroside E in six batches of samples were 0.130%-0.145%and 0.0708%-0.0776%.Conclusion Compared with the content determination methods in Pharmacopoeia of the People’s Republic of China,the new established method showed good stability and specificity,and short analysis time.It could be used for quality control study of Acanthopanacis Senticosi Radix et Rhizoma seu Caulis.

关 键 词：刺五加 刺五加苷B 刺五加苷E 超快速液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]