黄连解毒汤改善1-氯-2,4-二硝基苯诱导特应性皮炎小鼠瘙痒症状的作用及机制研究  

Study on the Effect and Mechanism of Huanglian Jiedu Decoction in Improving Itching Symptoms in1-chloro-2,4-dinitrobenzene-Induced Atopic Dermatitis Mice

作　　者：张曾燕 张汇源 关建华 陈云龙 ZHANG Zengyan;ZHANG Huiyuan;GUAN Jianhua;CHEN Yunlong(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学,天津301617

出　　处：《中药新药与临床药理》2023年第12期1713-1720,共8页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：天津市教委科研计划项目(2018KJ003)。

摘　　要：目的探讨黄连解毒汤改善1-氯-2,4-二硝基苯(DNCB)诱导特应性皮炎(AD)小鼠瘙痒症状的作用及机制。方法将36只Balb/c小鼠随机分为正常组、模型组、地塞米松组(阳性对照,2.5 mg·kg^(-1))及黄连解毒汤低、中、高剂量组(0.4、0.8、1.6 g·kg^(-1)),每组6只。剃除小鼠背部毛发后将200μL DNCB溶液涂抹于小鼠背部进行致敏(1%DNCB,连续3 d)与激发(1.5%DNCB,从第14天开始,每3 d激发1次,共进行5次)。激发与药物干预同时进行,各组按照设定剂量对小鼠进行灌胃给药,每日1次,连续14 d。参照特应性皮炎评分标准(SCORAD)计算小鼠皮损严重程度评分;记录小鼠20 min内抓挠次数。采用HE染色法观察皮损组织病理形态变化;甲苯胺蓝染色法观察皮损组织肥大细胞浸润情况;RT-q PCR法检测皮损组织中胸腺基质淋巴细胞生成素(TSLP)、白细胞介素(IL)13、组胺H4受体(HRH4)、IL-31 m RNA表达水平。结果与正常组比较,模型组小鼠的背部皮肤经DNCB激发后出现明显的红斑、苔藓化、结痂、表皮脱落等现象,皮损严重程度评分显著增高(P<0.001);表皮角化过度,棘层厚度显著增加(P<0.001),海绵水肿,真皮层内可见大量炎性细胞浸润;皮损组织肥大细胞数量显著升高(P<0.001);20 min内的抓挠次数显著增加(P<0.01);皮损组织中TSLP、IL-13、HRH4、IL-31 m RNA表达水平均明显升高(P<0.05,P<0.01)。与模型组比较,黄连解毒汤低、中、高剂量组小鼠背部的皮损情况得到明显改善,苔藓化面积明显缩小,皮损严重程度评分显著降低(P<0.001),皮损组织中肥大细胞数量及IL-13、HRH4、IL-31 m RNA表达水平均显著降低(P<0.05,P<0.01,P<0.001);黄连解毒汤中、高剂量组棘层厚度显著降低(P<0.001),真皮层内炎性细胞数量显著减少;黄连解毒汤高剂量组小鼠20 min内的抓挠次数显著减少(P<0.01),皮损组织中TSLP m RNA表达水平明显降低(P<0.05)。结论黄连解毒汤能够缓解AD小鼠瘙痒症�Objective To investigate the effect and mechanism of Huanglian Jiedu Decoction in improving the itching symptoms of 1-chloro-2,4-dinitrobenzene(DNCB)-induced atopic dermatitis(AD)in mice.Methods Thirty-six Balb/c mice were randomly divided into normal group,model group,Dexamethasone(positive control,2.5 mg·kg^(-1))group and Huanglian Jiedu Decoction low-,medium-and high-dose groups(0.4,0.8 and 1.6 g·kg^(-1)),6 mice in each group.After shaving the back of the mice,200μL of DNCB solution was applied to the back of the mice for sensitisation(1% DNCB for 3 consecutive days)and excitation(1.5% DNCB,starting from the fourteenth day,excitation was performed once every 3 days for a total of 5 times).The stimulation and drug interventions were carried out simultaneously,and each group was administered by gavage at a set dose once daily for 14 days.The severity score of the skin lesions was calculated with reference to the Scoring Atopic Dermatitis(SCORAD),and the number of times the mice scratched within 20 minutes was recorded.The pathological changes of the lesions were observed by HE staining;mast cell infiltration was observed by toluidine blue staining;and the m RNA expression levels of thymic stromal lymphopoietin(TSLP),interleukin(IL)13,histamine H4 receptor(HRH4),and IL-31in the lesions were detected by RT-q PCR.Results Compared with the normal group,the dorsal skin of the mice in the model group showed obvious erythema,mossification,crusting and epidermal shedding after DNCB excitation,and the severity score of the lesions was significantly increased(P<0.001);the hyperkeratosis of epidermis,the thickness of spinous layer was significantly increased(P<0.001),sponge oedema,and a large number of inflammatory cells infiltration was seen in the dermis;and the number of mast cells was significantly increased(P<0.001);the times of scratches within 20 minutes was significantly increased(P<0.01);and the m RNA expression levels of TSLP,IL-13,HRH4,and IL-31 in the skin lesion tissue were all significantly elevated(P<0.05

关 键 词：黄连解毒汤 特应性皮炎 瘙痒症状 胸腺基质淋巴细胞生成素 组胺H4受体 白细胞介素13 白细胞介素31 小鼠 

分 类 号：R285.5[医药卫生—中药学]