六灵解毒丸抑制金黄色葡萄球菌致大鼠急性咽炎的作用机制研究  

Study on the Mechanism of Liuling Jiedu Pills Inhibiting Acute Pharyngitis Induced by Staphylococcus Aureus in Rats

作　　者：高雨嫣 卢金福[1,2] 吕高虹 王恒斌[3] 罗年翠 杨方钰 许惠琴 喻斌 狄留庆[1,4] 杜萍 GAO Yuyan;LU Jinfu;LYU Gaohong;WANG Hengbin;LUO Niancui;YANG Fangyu;XU Huiqin;YU Bin;DI Liuqing;DU Ping(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023 Jiangsu,China;Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica,Nanjing 210023 Jiangsu,China;LEI YUN SHANG Pharmaceutical Group Co.Ltd.,Suzhou 215009 Jiangsu,China;Jiangsu Engineering Research Center for Efficient Delivery System of TCM,Nanjing 210023 Jiangsu,China;Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029 Jiangsu,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药药效与安全性评价重点实验室,江苏南京210023 [3]雷允上药业集团有限公司,江苏苏州215009 [4]江苏省中药高效给药系统工程技术研究中心,江苏南京210023 [5]南京中医药大学附属医院,江苏南京210029

出　　处：《中药新药与临床药理》2023年第12期1736-1742,共7页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：江苏省科技成果转化资金项目(BA2016104)。

摘　　要：目的探讨六灵解毒丸对金黄色葡萄球菌致大鼠急性咽炎的作用及机制。方法采用大鼠咽喉部注入1×10^(9)CFU·mL^(-1)的金黄色葡萄球菌溶液的方法复制急性咽炎大鼠模型。将SD大鼠随机分为空白组、模型组、蓝芩口服液组(5 mL·kg^(-1))及六灵解毒丸低、中、高剂量组(4.375、8.750、17.500 mg·kg^(-1)),每组10只。各组大鼠灌胃给药,每日1次,连续7 d。造模及给药期间每天观察并记录大鼠一般情况,并对咽喉局部炎症情况进行评分;采用苏木素-伊红(HE)染色法观察大鼠咽部组织病理学变化;ELISA法检测大鼠血清白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)含量;免疫组化法及Western Blot法检测大鼠咽部组织中IL-1β、IL-6、TNF-α蛋白表达水平。结果与空白组比较,模型组大鼠咽喉红肿程度显著增加,炎症评分显著升高(P<0.01),造模后第5~7天的体质量显著降低(P<0.05,P<0.01),咽部组织病理学评分显著升高(P<0.01),血清炎症因子IL-1β、IL-6、TNF-α水平均显著上升(P<0.01),咽部组织中IL-1β、IL-6、TNF-α蛋白表达水平显著升高(P<0.01)。与模型组比较,蓝芩口服液组及六灵解毒丸低、中、高剂量组大鼠的咽喉红肿明显减轻,炎症评分显著降低(P<0.01),血清IL-1β、IL-6、TNF-α水平均显著下降(P<0.01);蓝芩口服液组及六灵解毒丸中、高剂量组大鼠造模后第7天的体质量显著增加(P<0.01),咽部组织病理学评分及IL-1β、IL-6、TNF-α蛋白表达水平均显著降低(P<0.05,P<0.01)。结论六灵解毒丸可显著改善急性咽炎大鼠的症状及咽部组织炎症病理改变,其机制可能与下调IL-1β、IL-6、TNF-α等炎性因子表达水平有关。Objective To investigate the effect and mechanism of Liuling Jiedu Pills on acute pharyngitis caused by Staphylococcus aureus in rats.Methods The rat model of acute pharyngitis was replicated using the method of injecting 1×10~9CFU·mL~(-1)of Staphylococcus aureus solution into the pharynx of rats.SD rats were randomly divided into a blank group,a model group,a Lanqin Oral Solution group(5 mL·kg~(-1)),and a low-,medium-,and highdose group of Liuling Jiedu Pills(4.375,8.750,and 17.500 mg·kg~(-1)),with 10 rats in each group.Rats in each group were administered the drug by gavage once a day for 7 days.The general conditions of the rats were observed and recorded every day during the modeling and drug administration periods,and the local inflammation in the pharynx was scored;histopathological changes in the pharynx of the rats were observed by hematoxylin-eosin(HE)staining;serum interleukin 1β(IL-1β),interleukin 6(IL-6),tumor necrosis factorα(TNF-α),and tumor necrosis factor-α(TNF-α)were detected by ELISA.Immunohistochemistry and Western Blot were used to detect the protein expression levels of IL-1β,IL-6 and TNF-αin rat pharyngeal tissue.Results Compared with the blank group,rats in the model group had significantly increased pharyngeal erythema,significantly higher inflammation scores(P<0.01),significantly lower body mass on days 5-7 after modeling(P<0.05,P<0.01),significantly higher pathological scores(P<0.01),significantly higher levels of the serum inflammatory factors IL-1β,IL-6,and TNF-α(P<0.01),and significantly higher pharyngeal tissues showed significantly higher levels of IL-1β,IL-6,and TNF-α proteins(P<0.01).Compared with the model group,the pharyngeal erythema was significantly reduced in the Lanqin Oral Solution group and the low-,medium-and high-dose groups of Liuling Jiedu Pills,and the inflammation scores were significantly reduced(P<0.01),and the serum levels of IL-1β,IL-6,and TNF-α were significantly reduced(P<0.01);the body mass of the rats in the Lanqin Oral Solution group,and i

关 键 词：六灵解毒丸 急性咽炎 金黄色葡萄球菌 白细胞介素1Β 白细胞介素6 肿瘤坏死因子Α 大鼠 

分 类 号：R285.5[医药卫生—中药学]