高效液相色谱法同时测定工作场所空气中6种苯胺类化合物  

Simultaneous determination of six aniline compounds in workplace air by high performance liquid chromatography

作　　者：蒙瑞波[1] 丘静静 戎伟丰[1] 谢威民 袁静 何嘉恒[1] 胡嘉雯 张子群[1] 林佐侃[1] 吴凡 吴邦华[1] MENG Ruibo;QIU Jingjing;RONG Weifeng;XIE Weimin;YUAN Jing;HE Jiaheng;HU Jiawen;ZHANG Ziqun;LIN Zuokan;WU Fan;WU Banghua(Guangdong Province Hospital for Occupational Disease Prevention and Treatment,Guangzhou,Guangdong 510300,China;不详)

机构地区：[1]广东省职业病防治院,广东广州510300 [2]广州医科大学公共卫生学院,广东广州511436 [3]广东药科大学公共卫生学院,广东广州510310

出　　处：《中国职业医学》2023年第4期441-446,共6页China Occupational Medicine

基　　金：广东省医学科学技术研究基金(20201126145338375)。

摘　　要：目的建立同时测定工作场所空气中6种苯胺类化合物(ADs)的高效液相色谱方法。方法采用GDH-1空气采样管采集空气中蒸气态和气溶胶态共存的苯胺、邻甲苯胺、N-甲基苯胺、间甲基苯胺、对甲基苯胺和N,N-二甲基苯胺等6种ADs,用含体积分数为1.00%氨水的甲醇溶液解吸和洗脱后,以C18色谱柱分离,二极管阵列检测器测定。结果本方法测定6种ADs的定量测定范围为0.19~253.50 mg/L,相关系数均为0.9999;最低检出浓度和最低定量浓度分别为0.02~0.06和0.04~0.19 mg/m^(3)[以采集15.0 L样品计算,样品解吸(洗脱)溶液体积以3.00 mL计算];平均解吸和洗脱效率分别为92.15%~104.41%(硅胶)和94.29%~104.29%(滤膜);批内相对标准偏差(RSD)分别为0.90%~9.72%(硅胶)和0.57%~6.96%(滤膜),批间RSD分别为2.03%~9.78%(硅胶)和2.50%~8.62%(滤膜);样品在室温下可保存7 d。结论本方法可用于工作场所空气中6种ADs的同时测定。Objective To establish a high performance liquid chromatography(HPLC)method for simultaneous determination of six aniline compounds(ADs)in workplace air.Methods GDH-1 air sampling tube was used to collect six co-existing ADs such as aniline,o-toluidine,N-methylaniline,m-methylaniline,p-methylaniline and N,N-dimethylaniline in the vapor and aerosol of workplace air.The samples were desorbed and eluted using a methanol solution containing 1.00%ammonia water,followed by separation on a C18 chromatographic column and detection using a diode array detector.Results The quantification range of the method was 0.19-253.50 mg/L,with the correlation coefficient of 0.9999 for all six ADs.The minimum detection range was 0.02-0.06 mg/m^(3),and the minimum quantitation range was 0.04-0.19 mg/m^(3)[both calculated for a 15.0 L sample with a desorption(elution)solution volume of 3.00 mL].The average desorption and elution efficiencies were 92.15%-104.41%(silica gel)and 94.29%-104.29%(filter membrane).The intra-assay relative standard deviation(RSD)ranged from 0.90%-9.72%(silica gel)and 0.57%-6.96%(filter membrane).The inter-assay RSD ranged from 2.03%-9.78%(silica gel)and 2.50%-8.62%(filter membrane).The samples were stable at room temperature for seven days.Conclusion This method can be used for the simultaneous determination of six ADs in workplace air.

关 键 词：工作场所 空气 苯胺 化合物 共存 硅胶 滤膜 高效液相色谱法 

分 类 号：R134.4[医药卫生—劳动卫生]