十字花科黑腐病菌中一个与生长及Co^(2+)耐受性相关的小RNA的鉴定  

Identification of a Small RNA Involved in Growth and Co^(2+)Tolerance in Xanthomonas campestris pv.campestris

作　　者：马洁 胡姗姗 唐东阶[1,2] MA Jie;HU Shanshan;TANG Dongjie(College of Life Science and Technology,Guangxi University,Nanning,530004;State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Nanning,530004)

机构地区：[1]广西大学生命科学与技术学院,南宁530004 [2]亚热带农业生物资源保护与利用国家重点实验室,南宁530004

出　　处：《基因组学与应用生物学》2023年第11期1214-1222,共9页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31860031)资助。

摘　　要：细菌小RNA(small RNA, sRNA)又称非编码RNA (non-coding RNA, ncRNA),是一组长度为50~500 nt的RNA分子,通常不编码蛋白质,但具有独立的功能。sRNA作为转录后调节因子在细菌的各种生理过程中发挥重要的作用。尽管目前人们已经从各种细菌中鉴定了大量小RNA,但是大多数小RNA的功能还不清楚。本实验室之前在十字花科黑腐病菌(Xanthomonas campestris pv.capestris, Xcc) 8004菌株(简称Xcc 8004)中鉴定了612个小RNA,但绝大多数小RNA的功能仍然未知。本研究采用过表达的方法对小RNA(sRNA-Xcc132)的生物学功能进行鉴定。结果发现,与带有空载质粒的野生型菌株相比,sRNA-Xcc132的过表达菌株OE132在基本培养基中的生长速度明显加快而且对Co^(2+)的耐受力明显增强,说明sRNA-Xcc132与Xcc 8004的生长和Co^(2+)耐受性有关。Bacterial small RNA(small RNA,sRNA),also known as non-coding RNA(non-coding RNA,ncRNA),is a group of RNA molecules that are 50~500 nucleotides in length,normally do not encode proteins,but has independent functions.sRNA acts as a post-transcriptional regulator and plays an important role in various bacterial cellular physiological processes.Although a large num-ber of sRNAs had been identified in dfferent bacterial species,few of them has been functionally characterized.Our laboratory pre-viously identified 612 sRNAs from Xanthomonas campestris pv.campestris strain 8004(Xcc 8004),but the function of most small RNAs is still unknown.In this study,the biological function of the sRNA(sRNA-Xcc132)has been determined by using overexpres-sion method.The results showed that the sRNA-Xcc132 overexpression strain OE132 displayed a significantly increased growth rate in minimal medium and a greatly enhanced tolerance to Co^(2+) compared to the wild-type strain carrying the empty vector plasmid,demon-strating that sRNA-Xcc132 is involved in growth and Co^(2+)tolerance of Xcc 8004.

关 键 词：十字花科黑腐病菌 小RNA 过表达 Co^(2+)耐受 生长 

分 类 号：S432[农业科学—植物病理学]