亮斑扁角水虻幼虫酶解制备蛋白胨工艺优化及蛋白胨性质  

Peptone prepared from Hermetia illucens larvae:optimization of enzymolysis conditions and characteristics

作　　者：马石霞 刘高强 冶秀燕 周筱力 李新鹏 刘文钊 丁功涛 陈士恩[2] MA Shixia;LIU Gaoqiang;YE Xiuyan;ZHOU Xiaoli;LI Xinpeng;LIU Wenzhao;DING Gongtao;CHEN Shi’en(China-Malaysia National Joint Laboratory,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,Gansu,China;Life Science and Engineering College,Northwest Minzu University,Lanzhou 730030,Gansu,China;College of Chemical Engineering,Northwest Minzu University,Lanzhou 730030,Gansu,China;Sichuan Chemical Works Group Limited,Chengdu 610000,Sichuan,China)

机构地区：[1]西北民族大学生物医学研究中心中国-马来西亚国家联合实验室,甘肃兰州730030 [2]西北民族大学生命科学与工程学院,甘肃兰州730030 [3]西北民族大学化工学院,甘肃兰州730030 [4]川化集团有限责任公司,四川成都610000

出　　处：《微生物学通报》2023年第12期5337-5349,共13页Microbiology China

基　　金：西北民族大学生物工程一流学科(81080334)。

摘　　要：【背景】用于餐厨垃圾等有机废弃物处理的亮斑扁角水虻(Hermetia illucens,HI)含有丰富的氨基酸和营养物质,是一种优质、经济的蛋白来源。【目的】利用亮斑扁角水虻幼虫(Hermetia illucens larvae,HIL)制备蛋白胨用于细菌培养,为亮斑扁角水虻的应用方式提供新的思路。【方法】以亮斑扁角水虻幼虫为原料,利用单因素试验确定最佳酶解条件,对比分析HIL蛋白胨和市售胰蛋白胨的基本性质和功能性状,并进行细菌培养、生物化学试验,利用两种蛋白胨分别培养大肠杆菌(Escherichia coli)ATCC 25922模式细菌并通过生长动力学分析评价应用效果。【结果】制备HIL蛋白胨的最佳酶解工艺为按1.3%(质量分数)添加复合酶(胰酶:碱性蛋白酶质量比为1:1),在pH 7.0、54℃条件下反应4 h,此时HIL的水解度为(19.34±0.15)%。HIL蛋白胨和市售胰蛋白胨的功能性状和生物化学试验差异不显著(P>0.05)。大肠杆菌ATCC 25922在HIL蛋白胨培养基中生长的Xmax和λ分别为6.44和2.45,在市售胰蛋白胨生长的Xmax和λ分别为6.14和3.19,差异均不显著(P>0.05)。【结论】在培养大肠杆菌时,HIL蛋白胨可以替代胰蛋白胨作为微生物培养基的成分。[Background]Hermetia illucens(HI)used for the treatment of organic wastes such as kitchen waste is rich in amino acids and nutrients,serving as a high-quality and economical source of protein.[Objective]To prepare peptone with H.illucens larvae(HIL)for bacterial culture and provide a new idea for the application of HI.[Methods]Peptone was prepared from HIL by enzymolysis.The optimal enzymolysis conditions were determined by single factor tests,and the biochemical and functional properties of HIL-derived peptone and commercial tryptone were compared.Escherichia coli ATCC25922 was cultured with two peptone products and the growth kinetics was compared.[Results]The optimal conditions for preparing HIL-derived peptone were enzymolysis with the enzyme complex(trypsin:alkaline protease=1:1)added at 1.3%(mass fraction)and at pH 7.0 and 54℃for 4 h,under which the hydrolysis degree of defatted HIL was(19.34±0.15)%.The functional and biochemical properties showed no significant differences between HIL-derived peptone and commercial tryptone(P>0.05).The Xmax,andλof E.coli ATCC 25922 grown in the medium supplemented with HIL-derived peptone were 6.44 and 2.45,respectively,and those of E.coli ATCC 25922 grown in the medium supplemented with commercial tryptone were 6.14 and 3.19,respectively.The Xmax,andλshowed no significant differences between HIL-derived peptone and commercial tryptone(P>0.05).[Conclusion]HIL-derived peptone can replace commercial tryptone as a component of the medium for culturing E.coli.

关 键 词：亮斑扁角水虻幼虫 蛋白胨 细菌培养 生长动力学 

分 类 号：S963[农业科学—水产养殖]