丝状真菌表面展示技术研究进展  

Research progress in surface display technology of filamentous fungi

作　　者：曾祥伟 邹瑶 周玉萍[1] 田长恩[1] ZENG Xiangwei;ZOU Yao;ZHOU Yuping;TIAN Chang’en(Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design,School of Life Sciences,Guangzhou University,Guangzhou 510006,Guangdong,China)

机构地区：[1]广州大学生命科学学院广东省植物适应性与分子设计重点实验室,广东广州510006

出　　处：《微生物学通报》2023年第12期5574-5587,共14页Microbiology China

基　　金：广东省自然科学基金(2020A1515011423)。

摘　　要：丝状真菌表面展示技术是将表达的目的蛋白固定在丝状真菌细胞表面的一项新兴基因工程技术。丝状真菌具有极强的蛋白质分泌能力和良好的蛋白质翻译后加工能力,因而越来越多的丝状真菌表面展示技术得到开发和应用。本文就丝状真菌表面展示系统的研发和应用进展进行综述,并介绍与该系统构建密切相关的丝状真菌的细胞壁组成、锚定蛋白和遗传转化方法等技术。Surface display technology,an emerging genetic engineering technology,can immobilize the target protein on the cell surface of filamentous fungi.Since filamentous fungi have strong abilities of protein secretion and post-translational processing,surface display technology has been developed for increasing filamentous fungi.We review the development and application of the surface display system of filamentous fungi and introduce the cell wall composition,anchored proteins,and genetic transformation methods of filamentous fungi which are closely related to the construction of the system.

关 键 词：丝状真菌 表面展示技术 糖基磷脂酰肌醇(glycosylphosphatidylinositol GPI)锚定蛋白 细胞壁 

分 类 号：Q93[生物学—微生物学] Q78