脑胶质瘤的MRI诊断及昼夜节律基因CRY1对脑胶质瘤的表达特性研究  

MRI Diagnosis of Glioma and the Expression Characteristics of Circadian Gene CRY1 on Glioma

作　　者：吴娟[1] 王棒 陈硕硕 王大巍 WU Juan;WANG Bang;CHEN Shuo-shuo;WANG Da-wei(Department of Neurosurgery,The Second Affiliated Hospital of Bengbu Medical College,Bengbu 233000,Anhui Province,China)

机构地区：[1]蚌埠医学院第二附属医院神经外科,安徽蚌埠233000

出　　处：《中国CT和MRI杂志》2023年第12期31-34,共4页Chinese Journal of CT and MRI

基　　金：安徽省重点科研项目(2022AH051507);安徽省重点科研项目(KJ2021A0786);蚌埠医学院研究生科研创新项目(Byycx21079)。

摘　　要：目的本项研究探讨脑胶质瘤的MRI诊断及昼夜节律基因CRY1对脑胶质瘤表达特性的研究。方法通过MRI等主要影像学诊断脑胶质瘤,片状弥散性T1低信号、T2高信号,可无明显强化为中枢神经系统肿瘤诊断时唯一需要影像学标准的疾病。基于GEO和TCGA数据库下载CRY1基因在胶质瘤中的表达数据。生存分析采用Kapian-Meier和Cox比例-风险回归。最小绝对收缩和选择算符(LASSO)筛选与生存相关的免疫细胞。RNA-Seq分析证实了结果。结果昼夜节律基因CRY1在脑胶质瘤中的高表达与较差的预后相关。GSEA软件筛选出的与胶质瘤相关的信号通路中参与上调的通路有7条。筛选与CRY1结合的蛋白,研究TCGA数据库中CRY1改变频率、突变类型。发现CRY1表达高水平和较高的免疫细胞浸润率相关,对免疫治疗的敏感性更高。结论CRY1的高表达水平与胶质瘤的不良预后密切相关,且CRY1具有较高的免疫浸润率,对免疫治疗的敏感性更高,则CRY1可能做为胶质瘤免疫治疗的生物标志物的潜在治疗靶点。Objective This study investigated the MRI diagnosis of glioma and the expression characteristics of the circadian gene CRY 1 on glioma.Methods The diagnosis of glioma by MRI and other major imaging,sheet diffusion T1 hypointense and T2 hyperintense,and no obvious enhancement is the only disease that requires imaging standards in the diagnosis of central nervous system tumors.The CRY1 gene expression data in glioma were downloaded based on the GEO and TCGA databases.Survival analysis was performed using Kapian-Meier and Cox ratio-risk regression.The minimum absolute shrinkage and selection operator(LASSO)screens for immune cells associated with survival.The RNA-Seq analysis confirmed the results.Results High expression of the circadian gene CRY1 in glioma is associated with worse prognosis.There are seven pathways involved in the signaling pathway associated with glioma.Proteins bound to CRY1 were screened to investigate the frequency of CRY1 alteration and type of mutation in the TCGA database.Found that high levels of CRY1 expression were associated with higher rates of immune cell infiltration,with higher sensitivity to immunotherapy.Conclusion The high expression level of CRY 1 is closely associated with the poor prognosis of glioma,and CRY1 has a high immune infiltration rate and higher sensitivity to immunotherapy,so CRY1 may be a potential therapeutic target for the biomarker of glioma immunotherapy.

关 键 词：脑胶质瘤 MRI CRY1 诊断 预后 免疫细胞浸润 生物标志物 免疫治疗靶点 

分 类 号：R651.1[医药卫生—外科学]