大孔吸附树脂法分离纯化白僵菌素  

Separation and Purification of Beauvericin by Macroporous Adsorption Resin

作　　者：周站云[1] 郭月玲[1] 周博 李敏[1] 彭亮[1] 王崔岩[1] ZHOU Zhanyun;GUO Yueling;ZHOU Bo;LI Min;PENG Liang;WANG Cuiyan(National Engineering Research Center of Microbial Medicine,Hebei Industry Microbial Metabolic Technology Innovation Center,NCPC New Drug Research&Development Co.,Ltd.,Shijiazhuang 052165,China)

机构地区：[1]微生物药物国家工程研究中心、河北省工业微生物代谢技术创新中心、华北制药集团新药研究开发有限责任公司,河北石家庄052165

出　　处：《化学与生物工程》2023年第12期39-43,共5页Chemistry & Bioengineering

基　　金：河北省科技研发平台建设专项(22567651H)。

摘　　要：采用大孔吸附树脂法分离纯化白僵菌素,对工艺条件进行了优化。结果表明,白僵菌素发酵液乙醇浸泡液经HZ801树脂吸附(上柱液酒精度40%、上柱流速2.0 BV·h^(-1))、80%乙醇解吸、乙酸乙酯萃取、3.0%~5.0%活性炭脱色、异丙醚结晶和重结晶,可得到HPLC含量高于98.5%的精粉。该工艺绿色环保,树脂和有机溶剂均可回收再利用,适合工业化生产。In this paper,we separated and purified beauvericin by macroporous adsorption resin,and optimized the process.The results show that the beauvericin refined powder with the HPLC content of more than 98.5%can be obtained by the steps as follows:separation and purification of ethanol immersing solution of beauvericin fermentation broth by HZ801 resin adsorption(ethanol volume fraction of testing solution of 40%,flow rate of 2.0 BV·h^(-1)),80%ethanol desorption,ethyl acetate extraction,3.0%-5.0%activated carbon decolorization,and isopropyl ether crystallization and recrystallization.This process is environmentally-friendly,and both resin and organic solvents can be recycled and reused,making it suitable for industrial production.

关 键 词：白僵菌素 大孔吸附树脂 分离纯化 乙酸乙酯 萃取 异丙醚 结晶 

分 类 号：TQ465[化学工程—制药化工] R284[医药卫生—中药学]