丹参-白术药对“异病同治”癌性厌食和深静脉血栓的网络药理学机制分析  

The network pharmacological mechanism of Danshen-Baizhu couplet medicinals in treating cancer anorexia and deep vein thrombosis based on the theory of treating different diseases with the same method

作　　者：亓晓彤 宋萌萌[2] 乔元勋 QI Xiaotong;SONG Mengmeng;QIAO Yuanxun

机构地区：[1]山东中医药大学,山东济南250355 [2]泰安市中医医院,山东泰安271000

出　　处：《中医临床研究》2023年第30期24-33,共10页Clinical Journal Of Chinese Medicine

基　　金：泰安市科技创新发展项目(政策引导类)(2022NS155)。

摘　　要：目的:采取网络药理学方法研究讨论丹参-白术药对“异病同治”癌性厌食(Cancer Related Anorexia,CA)和深静脉血栓(Deep Venous Thrombosis,DVT)的机制。方法:先基于分析平台筛选丹参、白术的有效化学成分,然后对丹参-白术药对有效成分的靶点进行预测,最后构建网络。从DisGeNET、GeneCards数据库筛选CA和DVT涉及的靶点,整合疾病与药物靶点,获得共有靶点。使用STRING 11.5和Cytoscape 3.8.2筛选出关键共有靶点。再应用Metascape对筛选出的关键共有靶点作富集分析。结合分子对接预测结合潜能。结果:共检索到丹参-白术化合物257个,最终筛选出72个有效成分,对应131个药物靶点;共检索到CA疾病靶点1630个与DVT疾病靶点1166个,整合疾病靶点与药物靶点,得到共有靶点59个;进一步使用STRING 11.5和Cytoscape 3.8.2分析蛋白质间的相互作用,得到关键共有靶点28个;通过Metascape进行基因本体论(GO)富集分析,获得799个生物过程、18个细胞组分和47个分子功能;通过京都基因与基因组百科全书(KEGG)通路富集分析共获得145条信号通路,相关信号通路有癌症、磷脂酰肌醇3激酶(Phosphatidylinositol 3-kinase,PI3K)-丝氨酸/苏氨酸蛋白激酶(Akt Serine/Threonine Kinase,AKT)、丝裂原活化蛋白激酶(Mitogen-Activated Protein Kinase,MAPK)、白细胞介素(Interleukin,IL)-17、肿瘤坏死因子(Tumor Necrosis Factor,TNF)、T细胞受体、酪氨酸蛋白激酶(Janus-Activated Kinase,JAK)-信号转导与转录激活因子(Signal Transducer and Activator of Transcription,STAT)信号通路、松弛素、C型凝集素受体以及相关程序性死亡配体1(Programmed Cell Death Protein Ligand 1,PD-L1)和程序性死亡受体1(Programmed Cell Death Protein 1,PD-1)等,主要与炎症反应和免疫调节相关。分子对接得出有效成分与关键共同靶点均具有较好的结合潜能的结论。结论:丹参-白术药对“异病同治”CA和DVT的主要机制涉及炎症反应和免Objective:To investigate the mechanism of Danshen(Salvia miltiorrhiza)-Baizhu(Rhizoma Atractylodis Macrocephalae)couplet medicinals in treating cancer anorexia(CA)and deep vein thrombosis(DVT)based on the theory of treating different diseases with the same method by network pharmacology.Methods:First of all,the effective chemical ingredients of Danshen and Baizhu were screened on TCMSP,and in the second place the targets of the effective ingredients of Danshen-Baizhu were predicted,and finally the network was constructed.In DisGeNET and GeneCards databases,disease and drug targets were integrated to obtain common targets.Key common targets were screened using STRING 11.5 and Cytoscape 3.8.2.Then Metascape was applied to enrich the selected key common targets.By molecular docking,binding potential was predicted.Results:A total of 257 compounds of Danshen-Baizhu couplet medicinals were retrieved,and 72 active ingredients were screened out,corresponding to 131 drug targets.A total of 1630 disease targets of CA and 1166 disease targets of DVT were retrieved.A total of 59 mutual targets were obtained by integrating disease targets and drug targets.Furthermore,STRING 11.5 and Cytoscape 3.8.2 were used to analyze the interaction between proteins,and 28 key targets were obtained.By GO enrichment analysis with Metascape,799 biological processes,18 cell ingredients and 47 molecular functions were obtained.By KEGG enrichment analysis,145 signaling pathways were obtained,the relevant signaling pathways included cancer,PI3K-Akt,MAPK,IL-17,TNF,T cell receptor,JAK-STAT signaling pathway,relain,C-type lectin receptor,and related PD-L1 and PD-1,which were mainly related to inflammatory response and immune regulation.The conclusion of molecular docking was that effective ingredients and key common targets had good binding potential.Conclusion:The study concludes that the main mechanism of Danshen-Baizhu couplet medicinals in treating CA and DVT based on the theory of treating different diseases with the same method involves inflam

关 键 词：丹参-白术 网络药理学 异病同治 癌性厌食 深静脉血栓 

分 类 号：R273[医药卫生—中西医结合]