基于UPLC-Q-Orbitrap-HRMS技术鉴定护心胆中异喹啉类生物碱成分  

Identification of Isoquinoline Alkaloids Components in Corydalis Suaveolens Hance Based on UPLC-Q-Orbitrap-HRMS Technique

作　　者：刘畅 周熙 罗辉泰 黄芳 吴惠勤 LIU Chang;ZHOU Xi;LUO Hui-tai;HUANG Fang;WU Hui-qin(Guangdong Provincial Engineering Research Center for Quality and Safety of Traditional Chinese Medicine,Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology,Institute of Analysis,Guangdong Academy of Sciences(China National Analytical Center,Guangzhou),Guangzhou 510070,China)

机构地区：[1]广东省科学院测试分析研究所(中国广州分析测试中心),广东省化学测量与应急检测技术重点实验室,广东省中药质量安全工程技术研究中心,广东广州510070

出　　处：《分析测试学报》2023年第12期1547-1557,共11页Journal of Instrumental Analysis

基　　金：广东省科学院打造综合产业技术创新中心行动资金项目(2022GDASZH-2022010110)。

摘　　要：应用超高效液相色谱-四极杆静电场轨道阱高分辨质谱法(UPLC-Q-Orbitrap-HRMS)分析并鉴定了护心胆(Corydalis suaveolens Hance)块根中的异喹啉类生物碱。护心胆甲醇提取物经Poroshell RRHD C_(18)(150 mm×2.1 mm,1.8μm)色谱柱分离,以乙腈-0.1%甲酸水为流动相进行梯度洗脱。在正离子模式下使用加热电喷雾离子源进行数据采集得到总离子流图。通过对色谱图中各色谱峰的精确质量数进行识别,结合对照品数据及相关文献分析二级高分辨质谱信息。在护心胆甲醇提取物中共鉴定出62个异喹啉类生物碱,包括15个原小檗碱型生物碱、15个四氢小檗碱类生物碱、14个苄基异喹啉类生物碱、3个阿朴菲类生物碱、6个普罗托品类生物碱、7个苯并菲啶类生物碱、2个二氢苯并菲啶类生物碱。该研究对护心胆中异喹啉类生物碱成分进行了较为全面系统的解析,可为后续药效物质基础研究及应用开发奠定基础。This study applied ultra performance liquid chromatography tandem quadrupole electro⁃static field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap-HRMS)to analyze and identify isoquinoline alkaloids in the roots of Corydalis suaveolens Hance.The methanol extract of Co⁃rydalis suaveolens processed with a Poroshell RRHD C_(18) column(150 mm×2.1 mm,1.8μm)with acetonitrile and 0.1%formic acid water solution as the mobile phase for gradient elution.The heated electrospray ion source(HESI)was adopted to collect the data in positive ionization modes.The chemical constituents were identified and confirmed by analyzing the mass data from the reference substances and literature.A total of 62 isoquinoline alkaloids were identified in the methanol extract of Corydalis suaveolens Hance,including 15 berberine-type alkaloids,15 tetrahydro berberine-type alkaloids,14 benzylisoquinoline alkaloids,3 aporphine alkaloids,6 protamine type alkaloids,7 benzene not pyridine type alkaloids,and 2 dihydrobenzene not pyridine type alkaloids.This study comprehensively and systematically analyzed the components of isoquinoline alkaloids in the Coryda⁃lis suaveolens Hance,and provided a reference for the subsequent study of material basis and devel⁃opment.

关 键 词：护心胆 UPLC-Q-Orbitrap-HRMS 异喹啉类生物碱 成分分析 

分 类 号：O657.63[理学—分析化学] R284.1[理学—化学]