溶剂诱导萃取/UPLC-MS/MS法检测农田土壤中20种除草剂  

Determination of Herbicides in Agricultural Soils by Solvent Induced Extraction Coupled with UPLC-MS/MS

作　　者：瞿德业[1,2] 王彩虹 陈静 朱梦晨[1,2] 杨明月 夏搏 柴文博 杨山泉 QU De-ye;WANG Cai-hong;CHEN Jing;ZHU Meng-chen;YANG Ming-yue;XIA Bo;CHAI Wen-bo;YANG Shan-quan(College of Geography and Environmental Science,Northwest Normal University,Lanzhou 730070,China;Gansu Key Laboratory of Resource Environment and Sustainable Development of Oasis,Lanzhou 730070,China)

机构地区：[1]西北师范大学地理与环境科学学院,甘肃兰州730070 [2]甘肃省绿洲资源环境与可持续发展重点实验室,甘肃兰州730070

出　　处：《分析测试学报》2023年第12期1631-1638,共8页Journal of Instrumental Analysis

基　　金：甘肃省教育厅高等学校创新基金(2020B-098);西北师范大学青年教师科研能力提升计划(NWNW-LKQN2020-05)。

摘　　要：采用优化的溶剂诱导萃取前处理方法联合超高效液相色谱-串联质谱(UPLC-MS/MS),建立了同时快速测定土壤中20种除草剂的方法。采用1.5 mL二氯甲烷为萃取剂,5 mL乙腈为提取剂,涡旋时间8 min进行提取,以ZORBAX Eclipse Plus C_(18)色谱柱(100 mm×2.1 mm,1.8μm)进行分离,0.1%甲酸水和乙腈为流动相,电喷雾离子源(ESI)正离子方式扫描。结果显示,20种除草剂在对应质量浓度范围内线性良好,相关系数均不小于0.9990,方法检出限为0.1~1.0μg/kg,平均回收率为77.6%~115%,相对标准偏差为0.40%~3.8%。该方法简便快捷、灵敏度高、有机溶剂用量少,适用于实际样品中20种除草剂的高通量检测。An optimized solvent-induced extraction pretreatment method combined with ultra-perfor⁃mance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was established for the si⁃multaneous and rapid determination of 20 herbicides in agricultural soil.In the experiment,1.5 mL of dichloromethane was used as extractant,5 mL of acetonitrile as extractant,and 8 min vortex time were used for extraction,and ZORBAX Eclipse Plus C_(18)column(100 mm×2.1 mm,1.8μm)were used for separation,0.1%formic acid water and acetonitrile were used as mobile phase,and scanned with ESI mode.The results showed that 20 herbicides had good linearity in the correspond⁃ing mass concentration ranges with correlation coefficients not less than 0.9990.The detection limit of the method ranged from 0.1-1.0μg/kg,the average recoveries were 77.6%-115%,and the rela⁃tive standard deviations were between 0.40%-3.8%.This method is simple,fast,sensitive,and re⁃quires less organic solvent,which is suitable for high-throughput detection of 20 herbicides in actual samples.

关 键 词：溶剂诱导萃取 超高效液相色谱-串联质谱 除草剂 农田土壤 

分 类 号：O657.7[理学—分析化学] X132[理学—化学]