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作 者:魏敏[1,2] 孙晓东 庞强强 周曼 李申 陈家路[1] WEI Min;SUN Xiao-dong;PANG Qiang-qiang;ZHOU Man;LI Shen;CHEN Jia-lu(Henan Vocational College of Nursing,Anyang 455000,China;Hainan Key Laboratory of Vegetable Biology,Haikou 571100,Chi-na;The Institute of Vegetables,Hainan Academy of Agricultural Sciences,Haikou 571100,China)
机构地区:[1]河南护理职业学院,河南安阳455000 [2]海南省蔬菜生物学重点实验室,海南海口571100 [3]海南省农业科学院蔬菜研究所,海南海口571100
出 处:《中药材》2023年第4期811-816,共6页Journal of Chinese Medicinal Materials
基 金:海南省自然科学基金项目(322QN423);海南省重点研发专项(ZDYF2022XDNY143);海南省农业科学院基本科研业务费专项资金项目(2020);菲利克斯·达帕雷·达科拉海南院士创新平台科研专项。
摘 要:目的:鉴定引起薄荷叶斑病的病原菌种类及室内筛选该病害的防治药剂。方法:应用组织分离法获得疑似病原菌菌株,用柯赫氏法则确定病原菌。结合形态学与分子生物学方法进行鉴定,然后使用菌丝生长速率法测定8种杀菌剂对该病原菌的室内毒力活性。结果:从新乡市感染了叶斑病的薄荷叶片上分离出11个纯菌株,致病性试验结果表明,分离得到的真菌菌株HN-BH-3为薄荷叶斑病的致病菌。通过对致病菌rDNA-ITS序列、TUB2与TEF-1α基因序列分析,结合形态学特征,鉴定菌株HN-BH-3为球黑孢菌Nigrospora sphaerica;8种药剂的筛选结果表明,多菌灵、苯醚甲环唑和甲基硫菌灵对球黑孢菌的EC_(50)值均小于1 mg/L,分别为0.0330、0.0415、0.7478 mg/L。结论:从染病薄荷叶分离的病原菌为球黑孢菌;多菌灵对球黑孢菌具有较好抑制效果。Objective:To identify the species of pathogen causing leaf spot disease of Mentha haplocalyx,to screen the control a⁃gents of this disease in laboratory.Methods:The suspected pathogenic strain was obtained by tissue separation and identified by Koch′s rule.Morphological and molecular biological methods were used to identify the pathogen,and the mycelium growth rate method was used to determine the indoor virulence activity of 8 fungicides against the pathogen.Results:Eleven pure strains were isolated from the leaves of Mentha haplocalyx infected with leaf spot disease in Xinxiang city,and the pathogenicity test results showed that the isolated strain HN⁃BH⁃3 was the pathogenic pathogen of Mentha haplocalyx leaf spot disease.The strain HN⁃BH⁃3 was identified as Nigrospora spha⁃erica by the sequence analysis of rDNA⁃ITS sequence,TUB2 and TEF⁃1αgenes,combined with morphological characteristics.The screening results of 8 agents showed that EC_(50) values of carbendazim,difenoconazole and thiophanate⁃methyl against Nigrospora sphaeri⁃ca were less than 1 mg/L,which were 0.0330,0.0415,0.7478 mg/L,respectively.Conclusion:The pathogen isolated from infected Mentha haplocalyx leaves is Nigrospora sphaerica.Carbendazim has a good inhibition effect on Nigrospora sphaerica.
分 类 号:S432.4[农业科学—植物病理学]
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