基于UPLC-Q-TOF-MS技术的紫丁香化学成分及入血成分研究  

Study on the Chemical Constituents and Components into Blood of Syringa oblata Based on UPLC⁃Q⁃TOF⁃MS

作　　者：蔡定吉 李志强 彭万钱 张皓男 何明珍[1] 冯育林 钟国跃[1] CAI Ding-ji;LI Zhi-qiang;PENG Wan-qian;ZHANG Hao-nan;HE Ming-zhen;FENG Yu-lin;ZHONG Guo-yue(National Engineering Research Center for Manufacturing Technology of Solid Preparations of Traditional Chinese Medicine,Jiangxi University of Chinese Medicine,Nanchang 330006,China;State Key Laboratory of Innovation Medicine and High Efficiency and En-ergy Saving Pharmaceutical Equipment,Nanchang 330006,China)

机构地区：[1]江西中医药大学中药固体制剂制造技术国家工程研究中心,江西南昌330006 [2]创新药物与高效节能降耗制药设备国家重点实验室,江西南昌330006

出　　处：《中药材》2023年第4期892-902,共11页Journal of Chinese Medicinal Materials

基　　金：国家重点研发计划课题(2019YFC1712302,2019YFC1712304);江西省教育厅科技计划项目(GJJ201222);江西省科技创新平台项目(20194AFD45001);中央引导地方科技发展专项项目(20192ZDD02002);江西中医药大学校级创新创业训练计划项目(202110412213)。

摘　　要：目的:研究紫丁香的化学成分及大鼠灌胃给药紫丁香后大鼠血清中的入血成分。方法:采用UPLC-Q-TOF-MS技术,色谱条件为Welch XB-C18色谱柱(100 mm×2.1 mm, 1.8μm);流动相:0.1%甲酸水溶液-乙腈,梯度洗脱;流速:0.3 mL/min;柱温:40℃;进样量:2μL。通过对照品比对、结合文献数据及MassBank数据库信息,对紫丁香的化学成分及入血成分进行表征。结果:从紫丁香提取物中推测出38个化学成分,主要为木脂素和倍半萜类成分;从灌胃后大鼠血清中推测出40个入血成分,包括14个原型成分和26个代谢产物。结论:该实验运用UPLC-Q-TOF-MS技术及中药血清药物化学方法,快速分析了紫丁香的化学成分及入血成分,初步探究了其可能的药效物质基础,为紫丁香的进一步开发利用提供参考依据。Objective:To study on the chemical constituents ofSyringa oblataand the components into blood after intragastric ad⁃ministration ofSyringa oblatain rats.Methods:UPLC⁃Q⁃TOF⁃MS was performed on a Welch XB⁃C18column(100 mm×2.1 mm,1.8μm),the mobile phase was 0.1%formic acid solution⁃acetonitrile for gradient elution,the flow rate was 0.3 mL/min,column tempera⁃ture was 40℃,injection volume was 2μL.The chemical components and components into blood ofSyringa oblatawere characterized by comparison of reference materials,literature data and MassBank database information.Results:Thirty⁃eight chemical constituents were found from the extract ofSyringa oblata,including lignans and sesquiterpenes;40 components were found from rat serum after intragas⁃tric administration,including 14 prototype components and 26 metabolites.Conclusion:In this study,UPLC⁃Q⁃TOF⁃MS technology and serum pharmacochemical method of traditional Chinese medicine are used to rapidly analyze the chemical components and components into blood ofSyringa oblata,and preliminarily explore the possible pharmacodynamic substance basis ofSyringa oblata,which provide a reference for further development and utilization ofSyringa oblata.

关 键 词：紫丁香 UPLC-Q-TOF-MS 化学成分 入血成分 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]