基于UPLC-Q-TOF/MS的新月蕨属三种中药材化学成分分析  

Chemical Constituents of Three Traditional Chinese Medicinal Materials in Pronephrium Genus by UPLC⁃Q⁃TOF/MS

作　　者：汪依兰 徐箐 范先平 汪波 许沛虎[1] 肖凌 WANG Yi-lan;XU Jing;FAN Xian-ping;WANG Bo;XU Pei-hu;XIAO Ling(Wuhan University of Technology,Wuhan 430070,China;Hubei Institute for Drug Control/NMPA Key Laboratory of Quality Con-trol of Chinese Medicine/Hubei Engineering Research Center for Drug Quality Control,Wuhan 430075,China)

机构地区：[1]武汉理工大学,湖北武汉430070 [2]湖北省药品监督检验研究院/国家药品监督管理局中药质量控制重点实验室/湖北省药品质量检测与控制工程技术研究中心,湖北武汉430075

出　　处：《中药材》2023年第4期903-910,共8页Journal of Chinese Medicinal Materials

基　　金：湖北省重点研发计划项目(2020ACA007-01);湖北省青年拔尖人才培养计划(鄂组函[2021]10号)。

摘　　要：目的:对新月蕨属3种中药材的主要化学成分进行定性研究,分析三者差异成分;并对披针新月蕨的根茎和须根化学成分进行比较,评价药用部位。方法:色谱分离采用ACQUITY UPLC BEH Shield RP18色谱柱,甲醇-0.1%甲酸水溶液为流动相梯度洗脱,质谱采用超高效液相-四极杆-飞行时间串联质谱法(UPLC-Q-TOF/MS)及电喷雾(ESI)离子源,在正、负离子模式下采集数据。采用OPLS-DA筛选披针新月蕨根茎及须根的差异性成分。结果:应用Masslynx 4.1软件及The Critical Assessment of Small Molecule Identication(CASMI)方法鉴定了3种药材中的33种主要化学成分,其中披针新月蕨22种,红色新月蕨23种,二者共有成分20个,主要为黄酮类化合物;单叶新月蕨仅鉴定了8种,初步判断含有较多有机酸类化合物。披针新月蕨根茎及须根的差异性成分为6″-O-Acetyltriphyllin A、5,2′,5′-三羟基-7-甲氧基黄烷酮等8个化合物。结论:该研究初步阐明了新月蕨属3种中药材的药效物质基础,为制定3种药材的质量评价标准,明确质控指标奠定了基础。Objective:To analyze the chemical constituents from 3 kinds of traditional Chinese medicinal materials ofPronephrium and analyze the different components,to compare the chemical constituents of rhizomes and fibrous roots ofPronephrium penangianum and evaluate the medicinal parts.Methods:An ACQUITY UPLC BEH Shield RP18 column was used,mobile phase was methanol⁃0.1%formic acid aqueous solution with gradient elution.Mass spectrometry was performed by UPLC⁃Q⁃TOF/MS and electrospray(ESI)ion source.Data were collected in positive and negative ion modes.OPLS⁃DA was used to screen the different components in the rhizomes and fibrous roots ofPronephrium penangianum.Results:33 main components of three traditional Chinese medicinal materials were isola⁃ted and identified by using Masslynx 4.1 software and the Critical Assessment of Small Molecule Identification(CASMI)method,there were 22 compounds fromPronephrium penangianum,23 compounds fromPronephrium lakhimpurense,which were mainly flavonoids.There were 20 components in the two.Pronephrium simplexidentified only 8 compounds,preliminary judgment contained more organic acid compounds.There were 8 differential components in rhizome and fibrous roots ofPronephrium penangianum,including 6″⁃O⁃Acetyl⁃triphyllin A,5,2′,5′⁃trihydroxy⁃7⁃methoxyflavanone and others.Conclusion:This study preliminarily clarifies the pharmacodynamic ma⁃terial basis of 3 kinds of traditional Chinese medicinal materials ofPronephrium,which lays a foundation for the formulation of the quali⁃ty evaluation criteria of 3 kinds of traditional Chinese medicinal materials and the definition of the quality control index.

关 键 词：披针新月蕨 红色新月蕨 单叶新月蕨 超高效液相色谱-四极杆-飞行时间串联质谱法 偏最小二乘法-判别分析 CASMI 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]