住宅厨房给水管网末梢微生物多样性分析  

Analysis of Microbial Diversity at the End of Water Supply Pipelines Network in Residential Kitchens

作　　者：高将[1,2] 张宝军 孙悦[1,3] 袁涛 GAO Jiang;ZHANG Baojun;SUN Yue;YUAN Tao(Institute of Building Intelligence,Jiangsu Vocational Institute of Architectural Technology,Xuzhou 221116,China;School of Environment and Spatial Informatics,China University of Mining and Technology,Xuzhou 221116,China;Xuzhou Water Treatment Engineering Technology Research Center,Xuzhou 221116,China)

机构地区：[1]江苏建筑职业技术学院建筑智能学院,江苏徐州221116 [2]中国矿业大学环境与测绘学院,江苏徐州221116 [3]徐州市水处理工程技术研究中心,江苏徐州221116

出　　处：《净水技术》2023年第12期63-71,共9页Water Purification Technology

基　　金：徐州市重点研发计划项目(KC19221);徐州市应用基础研究计划项目(KC19049);徐州市基础研究计划青年科技人才项目(KC23036)。

摘　　要：采集住宅厨房给水管网龙头、枝状管网及净水器3个部位水样,采用蛋白酶K裂解法进行基因组DNA抽提,基于宏基因组学技术分析其微生物群落结构、代谢功能、抗生素抗性基因(ARGs)及毒力因子,探究给水支管末梢饮水安全风险。结果表明,住宅厨房给水管网末梢不同部位可能存在多种微生物,并且具备不同的特定功能,碳水化合物代谢是住宅厨房给水管网末梢样品中KEGG注释到数量最多的通路;adeF基因是最佳抗生素耐药性本体(ARO)抗性基因;进攻型毒力因子是住宅厨房给水管网末梢细菌毒力的主要类型;给水支管末梢附件更便于微生物富集,同时也存在较高的ARGs污染风险。研究可为住宅厨房给水管网末梢微生物多样性研究提供指导依据,为生活饮用水生物安全保障提供新思路。Collecting water samples from the tap,branch pipelines network and water filter of residential kitchen water supply pipelines network,extracting genomic DNA by proteinase K cleavage method,and analyzing the microbial community structure,metabolic function,antibiotic resistance genes(ARGs)and virulence factors based on petagenomics,the safety risk of drinking water at the end of water supply branch pipelines were explored.The results indicated that there would be multiple microorganisms presented in different parts of the residential kitchen water supply network and had different specific functions,carbohydrate metabolism was the pathway with the highest number of KEGG annotations in the residential kitchen water supply network endings samples,the adeF gene was the optimal antibiotic resistance ontology(ARO)resistance gene,and the aggressive virulence factor was the main type of bacterial virulence,the end attachment of water supply branch pipelines was more convenient for microbial enrichment and had a higher risk of antibiotic resistance genetic pollution.This study can provide guidance for the study of microbial diversity at the ends of residential kitchen water supply networks,and provide new ideas for ensuring the biological safety of drinking water.

关 键 词：给水管网 宏基因组学 微生物群落结构 抗生素抗性基因(ARGs) 毒力因子 

分 类 号：TU991[建筑科学—市政工程]