BiOBr/Ti_(3)C_(2) Mxene涂层赋予PEEK植入体声动力抗菌性能研究  

Sonodynamic antibacterial application of PEEK coated with BiOBr/Ti_(3)C_(2) Mxene

作　　者：吴自翔 孙学超 王茜[2] 杨至恒 刘锦隆 邓怡[1] 张龙[4] WU Zixiang;SUN Xuechao;WANG Qian;YANG Zhiheng;LIU Jinlong;DENG Yi;ZHANG Long(School of Chemical Engineering,Sichuan University,Chengdu 610065,China;College of Biomedical Engineering,Sichuan University,Chengdu 610065,China;College of Life Sciences,Sichuan University,Chengdu 610065,China;West China Second University Hospital,Sichuan Unversity,Chengdu 610065,China)

机构地区：[1]四川大学化学工程学院,成都610065 [2]四川大学生物医学工程学院,成都610065 [3]四川大学生命科学学院,成都610065 [4]四川大学华西第二医院,成都610065

出　　处：《功能材料》2023年第12期12029-12036,共8页Journal of Functional Materials

基　　金：国家自然科学基金委员会青年科学基金(32271392)。

摘　　要：骨缺损问题严重威胁着人们的生命健康。通过植入骨修复材料聚醚醚酮(PEEK)是治疗骨缺损问题的一种有效方法。然而,由于PEEK无抗菌活性而引起的感染制约了其在临床上的广泛应用。故本项目合成具有声动力性能的BiOBr/Ti_(3)C_(2)Mxene(下称BTC),通过1,3-二苯基异苯并呋喃(DPBF)消耗,罗丹明B(RhB)消耗,谷胱甘肽(GSH)消耗等方式检验了其声动力性能,再将其涂敷在磺化PEEK上,组成SP@BTC材料。接着选取大肠杆菌(E.coli)及金黄色葡萄球菌(S.aureus)来分别代表革兰氏阴性菌与革兰氏阳性菌,通过稀释涂布平板、细菌活性氧(ROS)检测、细菌形貌观察等方式以探究材料抗菌性能与机理。最终结果表明,BTC具有优良声动力性能,进而使得SP@BTC具有优良抗菌性能。研究探索了BTC材料在声动力领域的应用,同时为解决PEEK细菌感染问题提供了新思路。Bone defects seriously threaten people's lives and health,and implantation of the bone repair material polyetheretherketone(PEEK)is an effective way to treat it.However,the infection caused by PEEK's lack of antibacterial activity impedes its wide clinical adoption.Therefore,BiOBr/Ti_(3)C_(2) Mxene(hereinafter referred to as BTC)with sonodynamic properties were synthesized in this project,and its sonodynamic properties were verified by 1,3-diphenylisobenzofuran(DPBF)consumption,Rhodamine B(RhB)consumption,and glutathione(GSH)consumption.And then we coated it on sulfonated PEEK to form SP@BTC materials.We employed Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)to represent Gram-negative and Gram-positive bacteria respectively to explore the antibacterial properties and mechanism of SP@BTC by spread plate method,bacterial reactive oxygen species(ROS)detection and bacterial morphology observation.The final results show that BTC has excellent sonodynamic performance,which in turn makes SP@BTC have excellent antibacterial properties.This study explores the application of BTC material in the field of sonodynamic,and provides new ideas for solving the problem of bacterial infection of PEEK.

关 键 词：BiOBr PEEK 声动力 抗菌 植入体感染 

分 类 号：TB34[一般工业技术—材料科学与工程]