沙苑子总黄酮显色条件及提取工艺优选研究  

Optimization study on chromogenic conditions and extraction process of total flavonoids from Astragalus complanatus

作　　者：李婷 汪小玉 税丕先 张家伟 石厚银 沈骅睿 LI Ting;WANG Xiaoyu;SHUI Pixian;ZHANG Jiawei;SHI Houyin;SHEN Huarui(School of Pharmacy,Southwest Medical University,Sichuan,Luzhou 646000,China;Department of Clinical Research,West China Medical Technology Transfer Center,Sichuan,Chengdu 610041,China;Preparation Department,Sichuan Aupone Pharmaceutical Co.,Ltd.,Sichuan,Chengdu 610000,China;Department of Orthopedics and Traumatology,the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University,Sichuan,Luzhou 646000,China)

机构地区：[1]西南医科大学药学院,四川泸州646000 [2]西部医药技术转移中心临床研究部,四川成都610041 [3]四川奥邦古得药业有限公司制剂部,四川成都610000 [4]西南医科大学附属中医医院骨伤科,四川泸州646000

出　　处：《中国医药科学》2023年第23期73-76,共4页China Medicine And Pharmacy

基　　金：泸州市人民政府-西南医科大学科技战略合作项目(2021LZXNYD-J31);四川省中医药管理局中医药科研专项课题(2021MS463)。

摘　　要：目的优选沙苑子总黄酮显色及提取工艺。方法以沙苑子饮片为原料,通过单因素结合正交试验法,研究沙苑子总黄酮的显色方法及最佳显色条件、提取方法及最佳提取工艺。结果以AlCl_(3)法为宜;最佳显色条件为0.8 ml提取液加入1.2 ml 60%乙醇,2%AlCl_(3)0.6 ml、pH 4.5的醋酸-醋酸钠缓冲液2.0 ml,60%乙醇定容,室温静置20 min,于420 nm处测定吸光度;最佳提取工艺为70%乙醇,85℃加热回流提取2 h,料液比1∶25(g/ml),提取2次,得总黄酮的含量为24.5 mg/g。结论该显色和提取方法可行性高,重复性较好,本研究为沙苑子的进一步开发利用提供了实验依据。Objective To optimize the chromogenic and extraction process of total flavonoids from Astragalus complanatus.Methods By using Astragalus complanatus decoction pieces as raw materials,the chromogenic method,optimal chromogenic conditions,extraction method and optimal extraction process of total flavonoids from Astragalus complanatus were studied by single factor combined with orthogonal test.Results AlCl_(3)method was suitable.The optimal chromogenic conditions were as follows:add 1.2 ml of 60%ethanol,0.6 ml of 2%AlCl_(3)and 2.0 ml of pH 4.5 acetic acid-sodium acetate buffer to 0.8 ml of the extract,dilute with 60%ethanol to volume,let it stand at room temperature for 20 min,and measure the absorbance at 420 nm.The optimal extraction process was set as follows:70%ethanol,reflux extraction at 85℃for 2 h,solid-liquid ratio of 1∶25(g/ml),2 extractions in total,and the content of total flavonoids obtained was 24.5 mg/g.Conclusion The chromogenic and extraction method is feasible and reproducible.Therefore,this study provides experimental basis for the further development and utilization of Astragalus complanatus.

关 键 词：沙苑子 总黄酮 显色条件 提取工艺 

分 类 号：R284.2[医药卫生—中药学]