当归鸡血藤汤对再生障碍性贫血模型大鼠骨髓造血功能的影响  

Research regarding the way Danggui Jixueteng decoction affects the hematological function of the bone marrow in rats with aplastic anemia

作　　者：郭明鑫 李文静[3] 孙瑶湘 胡志强 沈颖 许全平 GUO Ming-xin;LI Wen-jing;SUN Yao-xiang;HU Zhi-qiang;SHEN Ying;XU Quan-ping(Department of Pharmacy,The Afiliated Yixing Hospital of Jiangsu University,Yixing 214200,Jiangsu Province,China;Clinical Laboratory,The Afiliated Yixing Hospital of Jiangsu University,Yixing 214200,Jiangsu Province,China;School of Pharmacy,Qiqihar Medical University,Qiqihaer 161003,Heilongjiang Province,China)

机构地区：[1]江苏大学附属宜兴医院药学部,江苏宜兴214200 [2]江苏大学附属宜兴医院检验科,江苏宜兴214200 [3]齐齐哈尔医学院药学院,黑龙江齐齐哈尔161003

出　　处：《中国临床药理学杂志》2023年第23期3434-3438,共5页The Chinese Journal of Clinical Pharmacology

基　　金：江苏省自然科学基金青年基金资助项目(BK20210074);宜兴市科技攻关指导基金资助项目(20221612)。

摘　　要：目的 探讨当归鸡血藤汤(DJD)对再生障碍性贫血(AA)模型大鼠骨髓造血功能的影响。方法 将SD大鼠随机分为空白组(0.9%NaCl)、模型组(0.9%NaCl)、阳性对照组(10.8 g·kg^(-1)复方阿胶浆)和低、中、高剂量实验组(3.10、6.21和12.42 g·kg^(-1)DJD)。用乙酰苯肼联合环磷酰胺建立AA大鼠模型,造模的同时给药,每天1次,连续15 d。测定大鼠体质量、血常规及骨髓中造血因子水平;观察大鼠骨髓涂片,进行骨髓有核细胞计数。结果 中、高剂量实验组和阳性对照组、模型组、空白组的血常规中白细胞数量分别为(2.44±0.46)×10^(9)、(4.26±0.38)×10^(9)、(4.57±1.43)×10^(9)、(0.96±0.18)×10^(9)和(9.83±0.87)×10^(9)·L^(-1),红细胞计数分别为(3.14±0.61)×10^(12)、(3.44±0.35)×10^(12)、(4.06±0.86)×10^(12)、(1.35±0.54)×10^(12)和(9.61±1.18)×10^(12)·L^(-1),血红蛋白数量分别为(87.83±7.25)、(108.17±8.93)、(99.50±13.81)、(22.33±7.63)和(153.92±19.42)g·L^(-1),血小板数量分别为(218.50±28.05)×10^(9)、(291.83±32.32)×10^(9)、(269.33±67.60)×10^(9)、(37.50±12.21)×10^(9)和(507.83±181.01)×10^(9)·L^(-1),骨髓中粒细胞巨噬细胞集落刺激因子水平分别为(317.08±31.86)、(359.70±36.37)、(394.86±23.41)、(198.31±14.63)和(379.39±14.95)ng·L^(-1),血管内皮细胞黏附分子1水平分别为(765.98±60.32)、(877.96±24.75)、(812.74±66.40)、(588.25±58.15)和(929.01±35.50)μg·L^(-1),白细胞介素3水平分别为(129.42±6.19)、(121.31±12.65)、(124.24±2.50)、(83.24±11.05)和(135.67±7.66)ng·L^(-1),胱天蛋白酶3水平分别为(105.49±10.07)、(115.49±6.89)、(114.36±2.97)、(150.62±16.82)和(116.10±4.68)pmol·L^(-1),骨髓损伤评分分别为(1.31±0.18)、(1.96±0.09)、(1.49±20.42)、(3.76±0.19)和(0.05±0.04)分,有核细胞计数分别为(5.79±0.47)×10^(6)、(5.12±0.10)×10^(6)、(5.56±0.25)×10^(6)、(3.20±0.28)×10^(6)和(8.49±0.56)×10^(6)·L^(-1)。空白组的上述指标与模型组比较,中、高�Objective To investigate the effect of Danggui Jixueteng decoction(DJD)on bone marrow hematopoietic function in aplastic anemia(AA)model rats.Methods SD rats were randomly divided into blank group(0.9%NaCl),model group(0.9%NaCl),positive control group(10.8 g·kg^(-1)Fufang E Jiao Jiang),experimental-H,-M,-L groups(3.10,6.21,12.42 g·kg^(-1)DJD).AA rat model was established by acetophenylhydrazine combined with cyclophosphamide,and the model was administered at the same time,once a day for 15 days.Body mass,blood routine and hematopoietic factors in bone marrow were determined.Bone marrow smears of rats were observed and bone marrow nucleated cells were counted.Results The amounts of white blood cells in blood routine of experimental-M,experimental-H,positive control,model and blank groups were(2.44±0.46)×10^(9),(4.26±0.38)×10^(9),(4.57±1.43)×10^(9),(0.96±0.18)×10^(9) and(9.83±0.87)×10^(9)·L^(-1);the amounts of red blood cells were(3.14±0.61)×10^(12),(3.44±0.35)×10^(12),(4.06±0.86)×10^(12),(1.35±0.54)×10^(12)and(9.61±1.18)×10^(12)·L^(-1);the amounts of hemoglobin were(87.83±7.25),(108.17±8.93),(99.50±13.81),(22.33±7.63)and(153.92±19.42)g·L^(-1);the amounts of platelets were(218.50±28.05)×10^(9),(291.83±32.32)×10^(9),(269.33±67.60)×10^(9),(37.50±12.21)×10^(9) and(507.83±181.01)×10^(9)·L^(-1);granulocyte macrophage colony stimulating factor levels in bone marrow were(317.08±31.86),(359.70±36.37),(394.86±23.41),(198.31±14.63)and(379.39±14.95)ng·L^(-1);vascular endothelial cell adhesion molecule 1 levels were(765.98±60.32),(877.96±24.75),(812.74±66.40),(588.25±58.15)and(929.01±35.50)μg·L^(-1);interleukin 3 levels were(129.42±6.19),(121.31±12.65),(124.24±2.50),(83.24±11.05),(135.67±7.66)ng·L^(-1);Caspase-3 levels were(105.49±10.07),(115.49±6.89),(114.36±2.97),(150.62±16.82)and(116.10±4.68)pmol·L^(-1);the bone marrow injury scores were(1.31±0.18),(1.96±0.09),(1.49±20.42),(3.76±0.19)and(0.05±0.04);the numbers of nucleated cells were(5.79±0.47)×10^(6),

关 键 词：当归鸡血藤汤 骨髓 再生障碍性贫血 骨髓抑制 造血微环境 免疫功能 

分 类 号：R28[医药卫生—中药学]