养阴凉血方对过敏性紫癜患儿血清诱导内皮细胞损伤的保护作用  

Protective effect of Yangyin Liangxue recipe on endothelial cells induced by serum in children with Henoch-schnlein purpura

作　　者：何分 史艳平[2] 李霞[2] 赵珍珍 邱锐 赵爱霞 HE Fen;SHI Yanping;LI Xia;ZHAO Zhenzhen;QIU Rui;ZHAO Aixia(Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学,陕西咸阳712046 [2]西安市儿童医院,陕西西安710003

出　　处：《陕西中医》2024年第1期17-22,共6页Shaanxi Journal of Traditional Chinese Medicine

基　　金：陕西省自然科学基础研究计划项目(2022JM-534);陕西省中医药管理局科研项目(ZYJXG-L23007);西安市卫生健康委员会科研项目(2023QN14)。

摘　　要：目的:探讨养阴凉血方对过敏性紫癜(HSP)患儿血清诱导损伤后人脐静脉内皮细胞(HUVECs)的影响及作用机制。方法:以HUVECs为研究对象,用健康儿童血清和HSP患儿血清进行干预,同时加入不同浓度的养阴凉血方,分为空白对照组、正常对照组、HSP组及养阴凉血方(0.5、1 g/ml)组,各组于培养24 h后收集上清液。倒置显微镜下观察各组细胞形态。ELISA法测定各组HUVECs中上清液肿瘤坏死因子α(TNF-α)、白细胞介素-8(IL-8)、免疫球蛋白A(IgA)、FcαRI(CD89)含量。Western blot检测各组HUVECs中PI3K蛋白表达水平。结果:与空白对照组和正常对照组相比,HSP组TNF-α、IL-8、IgA、CD89含量及PI3K蛋白磷酸化水平明显升高(均P<0.05),镜下观察细胞数目减少、形态皱缩;与HSP组比较,两组给药组的TNF-α、IL-8、IgA、CD89含量及PI3K蛋白磷酸化水平明显降低(均P<0.05),镜下观察细胞数目减少、形态皱缩等趋势变差情况有所改善。结论:养阴凉血方对于过敏性紫癜有较好的治疗作用,其作用机制可能与调控细胞因子如TNF-α、IL-8、IgA、CD89的分泌和降低PI3K/AKT/NF-κB信号通路PI3K蛋白的磷酸化水平有关。Objective:To investigate the protective effect and mechanism of Yangyin Liangxue recipe on endothelial cells induced by serum of children with Henoch-schonlein purpura(HSP).Methods:HUVECs were used as the research object,the serum of healthy children and children with HSP were to intervene,and different concentrations of Yangyin Liangxue recipe were added.They were divided into blank control group,normal control group,HSP group and Yangyin Liangxue recipe(0.5,1 g/ml)group.The supernatant was collected after 24 hours of culture.Cell morphology was observed under inverted microscope.The levels of TNF-α,IL-8,IgA,CD89 in the supernatant of each group were determined by ELISA.Western blot was used to detect the expression of PI3K protein in each group.Results:The levels of TNF-α,IL-8,IgA,CD89 and the expression PI3K protein in the supernatant of each group were significantly higher in HSP group than in the blank and normal control group(all P<0.05),and the number of cells was decreased and the morphology was shrunk.Compared with the HSP group,the levels of TNF-α,IL-8,IgA,CD89 and PI3K protein expression in the Yangyin Liangxue recipe two groups were significantly decreased(all P<0.05),and the trend of cell number reduction and morphological shrinkage was improved.Conclusion:Yangyin Liangxue recipe can treat Henoch-schonlein purpura and its mechanism may be related to regulating the secretion of cytokines such as TNF-α,IL-8,IgA and CD89 and reducing the phosphorylation level of PI3K protein in PI3K/AKT/NF-κB signaling pathway.

关 键 词：过敏性紫癜 人脐静脉内皮细胞 养阴凉血方 炎症因子 免疫球蛋白 PI3K蛋白 

分 类 号：R554.6[医药卫生—血液循环系统疾病]