基于MAPK/ERK通路探讨益肾调经丸对大鼠多囊卵巢综合征的作用和机制研究  

Effect and mechanism of Yishen Tiaojing pill on polycystic ovarian syndrome rats based on MAPK/ERK pathway

作　　者：刘雯星 陈怡 黄缨 殷秀琴 周芸 LIU Wenxing;CHEN Yi;HUANG Ying;YIN Xiuqin;ZHOU Yun(Hubei University of Traditional Chinese Medicine,Wuhan 430065,China)

机构地区：[1]湖北中医药大学,湖北武汉430065 [2]湖北中医药大学附属荆州中医院,湖北荆州434000 [3]荆州市中医药研究所,湖北荆州434000 [4]荆州市中医医院,湖北荆州434000

出　　处：《陕西中医》2024年第1期23-28,共6页Shaanxi Journal of Traditional Chinese Medicine

基　　金：湖北省自然科学基金资助项目(2022CFB351);湖北省医学领军人才培养工程专项经费资助项目[(2019)47];湖北省荆州市中医药研究所自主选题资助项目(ZZXT2022Y11)。

摘　　要：目的:探讨益肾调经丸对大鼠多囊卵巢综合征模型的作用及其机制。方法:70只雌性SD大鼠随机分为正常组、模型组、空白对照组、达英35组和益肾调经丸低、中、高剂量组,每组10只。采用来曲唑+高脂饲料造模,造模成功后,各治疗组和空白对照组使用相应剂量的药物灌胃28 d。ELISA法检测血清睾酮(T)、促卵泡素(FSH)、促黄体生成素(LH)水平,计算LH/FSH;观察卵巢形态改变;HE染色法观察卵巢镜下观;Real-time PCR法检测卵巢组织中CYP17A1、CYP19A1 mRNA表达;Western blot检测p-ERK1/2的蛋白表达。结果:与正常组比较,模型组大鼠卵巢可见多囊样改变,血清T、LH水平升高,FSH降低,LH/FSH升高,卵巢呈多囊样改变,卵巢组织中CYP17A1 mRNA表达升高,CYP19A1 mRNA表达降低,p-ERK1/2蛋白表达升高(P<0.05);与模型组对比,空白对照组各检测指标无明显变化,各给药组大鼠卵巢多囊样改变缓解,血清T、LH水平降低,FSH升高(P<0.05),卵巢多囊样改变减轻,卵巢组织中CYP17A1 mRNA表达降低,CYP19A1 mRNA表达升高(P<0.05),治疗组p-ERK1/2蛋白表达水平有不同程度改善,其中益肾调经丸各治疗组的改善比较差异有统计学意义(P<0.05),达英35组的变化比较差异无统计学意义(P>0.05)。结论:益肾调经丸能够改善PCOS大鼠性激素水平,减轻卵巢多囊样改变。益肾调经丸可能是通过MAPK/ERK信号通路降低CYP17A1、上调CYP19A1表达,降低雄激素水平,从而改善PCOS大鼠卵巢多囊样改变及高雄激素状态。Objective:To explore the effect of Yishen Tiaojing pill on polycystic ovary syndrome(PCOS)model rats.Methods:70 female SD rats were randomly divided into normal group,model group,normal saline group,Diane 35 group,Yishen Tiaojing pill low,medium,and high groups,with 10 rats in each group.Using letrozole and high-fat feed for modeling,after successful modeling,each treatment group and normal saline group were treated with corresponding doses of medication for 28 days.ELISA method was used to detect serum T,FSH,LH levels,and LH/FSH was calculated.Observed changes in ovarian morphology.HE staining was used to observe the ovaries.Real time PCR detection of CYP17A1 and CYP19A1.Western blot was used to detect the protein expression of p-ERK1/2.Results:Compared with the normal group,polycystic changes can be observed in the ovaries of model group rats,serum T and LH levels increased,FSH decreased,LH/FSH increased,and the ovaries showed polycystic changes.The expression of CYP17A1 mRNA increased,CYP19A1 mRNA decreased,and p-ERK1/2 protein expression increased in ovarian(P<0.05).Compared with the model group,there was no significant change in all detection indicators in the normal saline group.Alleviation of polycystic ovary changes in rats of each treatment group,serum T and LH levels were reduced,FSH was increased(P<0.05),polycystic ovary changes were alleviated,CYP17A1 mRNA expression was reduced in ovarian,and CYP19A1 mRNA expression was increased(P<0.05).The p-ERK1/2 protein expression level in the treatment group was improved to varying degrees,The improvement of each treatment group with Yishen Tiaojing pill was statistically significant(P<0.05),while the change in the Diane 35 group was not statistically significant(P>0.05).Conclusion:Yishen Tiaojing pill can improve sexual hormone levels and alleviate polycystic ovary changes in PCOS rats.Yishen Tiaojing pill may reduce CYP17A1,upregulate CYP19A1 expression,and lower androgen levels through the MAPK/ERK signaling pathway,thereby improving polycystic ovary changes

关 键 词：多囊卵巢综合征 MAPK/ERK通路 益肾调经丸 补肾活血法 高雄激素血症 来曲唑 大鼠 

分 类 号：R711.75[医药卫生—妇产科学]