丹参酮ⅡA调节Fas/FasL信号通路对脂多糖诱导的牙髓干细胞增殖和凋亡的影响  

Impacts of tanshinone IIA on lipopolysaccharide induced proliferation and apoptosis of dental pulp stem cells by regulating the Fas/FasL signaling pathway

作　　者：武万超 韩宇环 李丽洁[1] WU Wanchao;HAN Yuhuan;LI Lijie(不详;Department of Stomatology,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050,China)

机构地区：[1]内蒙古医科大学附属医院口腔科,呼和浩特010050 [2]呼和浩特市口腔医院综合科,呼和浩特010000

出　　处：《实用医学杂志》2023年第24期3182-3187,3194,共7页The Journal of Practical Medicine

基　　金：内蒙古自治区卫生健康科技计划项目(编号:202202170)。

摘　　要：目的探讨丹参酮ⅡA(TanⅡA)调节脂肪酸合成酶(Fas)/脂肪酸合成酶配体(FasL)通路对脂多糖(LPS)诱导的牙髓干细胞增殖和凋亡的影响。方法鉴定从需正畸的18~20岁患者第三磨牙中分离的人牙髓干细胞(hDPSCs)。用低、中、高剂量TanⅡA处理LPS诱导的hDPSCs后,再用人重组FasL蛋白(rh FasL)干预高剂量TanⅡA作用后的LPS诱导的hDPSCs,检测hDPSCs增殖、凋亡、hDPSCs上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6水平及hDPSCs中增殖细胞核抗原(PCNA)、裂解的天冬氨酸特异性半胱氨酸蛋白酶-3(Cleaved Caspase-3)、Fas、FasL蛋白表达。结果成功分离出hDPSCs。TanⅡA可促进LPS诱导的牙髓干细胞增殖,抑制凋亡,上调PCNA蛋白表达,抑制TNF-α、IL-6水平及Cleaved Caspase-3、Fas、FasL蛋白表达,且呈剂量依赖性,rh FasL对LPS诱导的牙髓干细胞的影响与上述对应指标变化趋势相反(P<0.05);rh FasL减弱了高剂量TanⅡA对LPS诱导的牙髓干细胞增殖的促进以及对细胞凋亡的抑制作用。结论TanⅡA可能通过抑制Fas/FasL信号通路促进LPS诱导的hDPSCs增殖,抑制凋亡。Objective To investigate the impacts of tanshinoneⅡA(TanⅡA)on lipopolysaccharide(LPS)induced proliferation and apoptosis of dental pulp stem cells by regulating the fatty acid synthase(Fas)/fatty acid synthase ligand(FasL)signaling pathway.Methods Identification of human pulp stem cells(hDPSCs)isolated from the third molar of 18~20 years old patients requiring orthodontics.Lps-induced hDPSCs were treated with low,medium and high doses of TanⅡA,and then human recombinant FasL protein(rh FasL)was used to intervene the LPS-induced hDPSCs after high dose TanⅡA.Proliferation and apoptosis of hDPSCs,levels of tumor necrosis factor-α(TNF-α)and interleukin(IL)-6 in hDPSCs supernatant,proliferating cell nuclear antigen(PCNA),Cleaved aspartate-specific cysteine proteinase-3(Cleaved Caspase-3),Fas,FasL protein expression were detected.Results hDPSCs were successfully isolated.In a dose-dependent manner,Tan IIA promoted LPS-induced proliferation,inhibited apoptosis,up-regulated PCNA protein expression,and inhibited TNF-α,IL-6 level,Cleaved Caspase-3,Fas,and FasL protein expression.The effect of rh FasL on LPS-induced dental pulp stem cells was opposite to the above indexes(P<0.05).rh FasL attenuates the effect of high-dose TanⅡA on pro-liferation and apoptosis of LPS-induced dental pulp stem cells.Conclusion TanⅡA may promote LPS induced hDPSCs proliferation and inhibit apoptosis by inhibiting the Fas/FasL signaling pathway.

关 键 词：丹参酮ⅡA 脂肪酸合成酶/脂肪酸合成酶配体信号通路 牙髓干细胞 增殖 凋亡 

分 类 号：R285[医药卫生—中药学]