骨髓增生异常综合征患者骨髓γδT细胞及其亚群的水平及意义  

Levels and significance ofγδT cells and their subpopulations in the bone marrow of MDS patients

作　　者：奚睿汀 耿素霞 黄欣[2] 李敏明 邓程新 王玉连 黄励思 翁建宇[2] 杜欣 XI Ruiting;GENG Suxia;HUANG Xin;LI Minming;DENG Chengxin;WANG Yulian;HUANG Lisi;WENG Jianyu;DU Xin(不详;School of Medicine,South China University of Technology,Guangzhou 510006,China;Guangdong Provincial People's Hospital(Guangdong Academy of Medical Sciences),Southern Medical University,Guangzhou 510080,China)

机构地区：[1]华南理工大学医学院,广州510006 [2]南方医科大学附属广东省人民医院(广东省医学科学院)血液科,广州510080

出　　处：《实用医学杂志》2023年第24期3195-3199,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:82070128);广东省自然科学基金项目(编号:2021A1515011436,2023A1515010170)。

摘　　要：目的了解骨髓增生异常综合征(myelodysplastic syndrome,MDS)患者骨髓γδT细胞及其亚群的水平,探索MDS患者骨髓微环境的免疫缺陷状况。方法收集MDS患者治疗前后及正常供者骨髓样本,多色流式检测骨髓γδT细胞及亚群水平,并分析MDS患者治疗后各细胞亚群的变化。结果MDS患者骨髓γδT细胞和滤泡辅助性γδT细胞明显降低(P<0.05)。不同分化程度的γδT细胞,仅初始γδT细胞水平明显降低(P=0.037),中枢记忆、效应记忆和终末分化γδT细胞MDS患者与正常供者差异无统计学意义(P>0.05)。免疫检查点PD1和TIM3阳性的γδT细胞分别存在一定程度的降低和升高,但差异无统计学意义(P>0.05)。治疗后获得疗效患者的γδT细胞和初始γδT细胞水平较治疗前明显升高,效应记忆γδT细胞较治疗前明显降低(P<0.05),85.71%(6/7)的MDS患者γδ+TIM3+T细胞水平治疗后不同程度的降低。结论MDS患者骨髓微环境γδT细胞及其亚群的水平存在不同程度的异常,治疗有效的患者,异常的γδT细胞恢复,通过γδT细胞及亚群水平检测可了解MDS的免疫缺陷状态,有可能作为判断疗效的指标,并为抗肿瘤免疫疗法提供新的资料。Objective To investigate the levels ofγδT cells and their subpopulations in bone marrow(BM)of patients with myelodysplastic syndrome(MDS),it aims to explore the immune deficiency status of BM microenvi-ronment in MDS patients.Methods BM samples were collected from MDS patients before and after treatment,as well as from normal donors.Multicolor flow cytometry was utilized to detect bone marrowγδT cells and subpopulation levels.The changes of the T cell subsets after treatment were also analyzed.Results The levels of BMγδT cells and follicular helperγδT cells from MDS patients were significantly lower than those of normal donors(P<0.05).AmongγδT cells at different stages of differentiation,only the frequencies of naïveγδT cells from MDS patients decreased significantly(P=0.037),and there was no significant difference observed about central memory,effector memory,and terminally differentiatedγδT cells in MDS patients compared to normal donors(P>0.05).Although there was a slight decrease in PD1+γδT cells and an increase in TIM3+γδT cells,these differences were not statistically significant(P>0.05).In patients who achieved a curative effect,the proportions ofγδT cells and naiveγδT cells increased significantly after treatment,and the effector memoryγδT cells decreased significantly after treatment(P<0.05).After treatment,85.71%(6/7)of MDS patients showed a decrease inγδ+TIM3+T cell levels to varying degrees.Conclusions The levels ofγδT cells and their subpopulations in the BM microenvironment of patients with MDS exhibit varying degrees of abnormalities.However,in patients who receive effective treatment,these abnormalγδT cells can recover.By detecting the levels ofγδT cells and subpopulations,we can gain insights into the immune deficiency status of MDS.This information might serve as an indicator to assess treatment efficacy and provide valuable insights for anti-tumor immunotherapy.

关 键 词：骨髓增生异常综合征 ΓΔT细胞 滤泡辅助性γδT细胞 调节性γδT细胞 程序性死亡受体1 T细胞免疫球蛋白黏蛋白分子3 

分 类 号：R551[医药卫生—血液循环系统疾病]