HPLC法测定消炎利胆片中三种成分的含量  

Simultaneous Determination of Three Components in Xiaoyan Lidan Tablets by HPLC

作　　者：李篮[1] 曾志云[1] 姜玉梅[1] 谢佳琦[1] Li Lan;Zeng Zhiyun;Jiang Yumei;Xie Jiaqi(Hunan Chemical Vocational Technology College,Zhuzhou 412000,China)

机构地区：[1]湖南化工职业技术学院,湖南株洲412000

出　　处：《山东化工》2023年第22期154-157,共4页Shandong Chemical Industry

基　　金：湖南省教育厅科学研究项目(21C1100)。

摘　　要：目的:建立HPLC法测定消炎利胆片中迷迭香酸、穿心莲内酯和脱水穿心莲内酯的含量。方法:采用高效液相色谱法,色谱柱以十八烷基硅烷键合硅胶为填充剂;以乙腈为流动相A,0.1%甲酸溶液为流动相B,梯度洗脱;流速为1 mL/min;检测波长:225 nm;柱温为室温。结果:迷迭香酸、穿心莲内酯和脱水穿心莲内酯线性范围分别为3.5~350μg/mL(r=0.9998)、19.6~1960μg/mL(r=0.9998)、25.3~2530μg/mL(r=0.9997),加样回收率分别为92.4%(2.6%,n=6)、99.8%(3.1%,n=6)、100.2%(3.2%,n=6)。结论:所建立的方法专属性强,重复性好、耐受性好,可用于消炎利胆片中迷迭香酸、穿心莲内酯和脱水穿心莲内酯的含量测定。Objective:To establish a HPLC method for the determination of rosmarinic acid,Andrographolide and dehydrated Andrographolide in Xiaoyan Lidan tablets.Methods:High-performance liquid chromatography was used.Octadecylsilane bonded silica gel was used as the filling agent in the chromatographic column;Using acetonitrile as mobile phase A and 0.1%formic acid solution as mobile phase B,gradient elution;The flow rate is 1 mL/min;Detection wavelength:225 nm;The column temperature is room temperature.Results:The linear ranges of rosmarinic acid,Andrographolide and dehydrated Andrographolide were 3.5~350μg/mL(r=0.9998),19.6~1960μg/mL(r=0.9998),and 25.3~2530μg/mL(r=0.9997);The recovery rates were 92.4%(2.6%,n=6)、99.8%(3.1%,n=6)、100.2%(3.2%,n=6).Conclusion:The established method has strong specificity,good repeatability and good tolerance,which can be used for the determination of Andrographolide,dehydrated Andrographolide and rosmarinic acid in Xiaoyan Lidan tablets.

关 键 词：HPLC 消炎利胆片 迷迭香酸 穿心莲内酯 脱水穿心莲内酯 

分 类 号：TQ460[化学工程—制药化工]