不同贮存年份的大别山区野生花椒精油成分分析及抗氧化活性测定  

Different Storage Time on Composition Analysis and Antioxidant Activity of Essential Oils from Wild Zanthoxylum Bungeanum in Dabie Mountain Area

作　　者：叶兆伟[1,2] 陈卫 马晓辉 乔林 王海燕 郭磊磊[1] 陈琼 YE Zhao-wei;CHEN Wei;MA Xiao-hui;QIAO Lin;WANG Hai-yan;GUO Lei-lei;CHEN Qiong(School of Biology and Pharmaceutical Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,China;Dabie Mountain Forest-medicine Ecological Planting Engineering and Technology Research Center of Henan Province,Xinyang 464000,China)

机构地区：[1]信阳农林学院制药工程学院,河南信阳464000 [2]河南省大别山林药生态种植工程技术研究中心,河南信阳464000

出　　处：《化学试剂》2024年第1期76-82,共7页Chemical Reagents

基　　金：河南省重大科技专项项目(201111310900);信阳市创新应用专项项目(20190022);校级科技创新团队建设项目(XNKJTD-008)。

摘　　要：为了分析不同贮存年份的大别山区野生花椒精油成分,并测定不同贮存年份花椒所提取精油的抗氧化能力,采用顶空气相色谱-质谱联用(HS-GC-MS)技术对其挥发性成分进行分析;采用DPPH法测定花椒精油的抗氧化活性。结果表明,当年、贮存1年和贮存2年的花椒精油提取率分别为(2.3±0.17)、(2.0±0.14)、(1.8±0.25)mL/100 g;当年、贮存1年和贮存2年的花椒精油中分离有效成分分别为23、15和12种;花椒精油浓度为16%时对DPPH的清除率最高,当年、贮存1年和贮存2年的花椒精油清除率分别达77.3%、64.7%和52.5%。因此,不同贮存年份的大别山区野生花椒精油均具有抗氧化能力,抗氧化能力随着贮存年份的延长而降低。在食品、保健品和香料香精等行业中使用花椒精油时,尽量使用当年采收的花椒。To analyze the composition and determine antioxidant activity of essential oils from wild Zanthoxylum bungeanum in Dabie mountain area with different storage years,Headspace Gas Chromatography-Mass Spectrometry(HS-GC-MS)technology was selected to analyze the essential oils,components.The antioxidative activity was investigated with the ability of scavenging DPPH as an index.The results show that the extraction rate of essential oils from wild zanthoxylum bungeanum in Dabie mountain area is 2.3±0.17(mL/100 g),2.0±0.14(mL/100 g),1.8±0.25(mL/100 g)stored for current year,1 year and 2 year respectively.Twenty-three,fifteen,twelve active components were isolated from the essential oils of Zanthoxylum bungeanum stored for current year,1 year and 2 year respectively.The highest DPPH removal rate was achieved at a concentration of 16%,with a maximum removal rate of 77.3%,64.7%,52.5%respectively.Therefore,the essential oil of wild Zanthoxylum bungeanum in Dabie mountain area in different storage years has antioxidant capacity.The antioxidant capacity decreases with the extension of storage years.When using peppercorn essential oils in the food,health food and flavor and fragrance industries,try to use peppercorn harvested in the current year.

关 键 词：贮存年份 大别山区 野生花椒 精油 HS-GC-MS 抗氧化 DPPH法 

分 类 号：TQ[化学工程]