光学法血小板计数对采血后可逆性血小板聚集的解聚效果  

Depolymerization effect of optical platelet count on reversible platelet aggregation after blood collection

作　　者：邓晨霞[1] 梅燕萍[2] 张霞 黄宝山 添丹 曹梦婷 胡永奇 林勇平[3] 田礼军 DENG Chenxia;MEI Yanping;ZHANG Xia;HUANG Baoshan;TIAN Dan;CAO Mengting;HU Yongqi;LIN Yongping;TIAN Lijun(Tongren Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200336,China;Nanjing First Hospital,Nanjing Hospital Affiliated to Nanjing Medical University,Nanjing 210006,Jiangsu,China;Guangzhou First Hospital Affiliated to Guangzhou Medical University,Guangzhou 510120,Guangdong,China;Xuzhou Children's Hospital,Xuzhou 221002,Jiangsu,China)

机构地区：[1]上海交通大学医学院附属同仁医院,上海200336 [2]南京医科大学附属南京医院南京市第一医院,江苏南京210006 [3]广州医科大学附属第一医院,广东广州510120 [4]徐州市儿童医院,江苏徐州221002

出　　处：《检验医学》2023年第11期1087-1090,共4页Laboratory Medicine

基　　金：上海市科学技术委员会重点专科项目(22YJ1900900)。

摘　　要：目的探讨血液分析仪光学法血小板(PLT)计数对乙二胺四乙酸依赖性假性血小板减低(EDTA-PTCP)的自解聚效应是否适用于抗凝采血后立即检测时发生的可逆性PLT聚集。方法选取2021年12月—2022年8月上海交通大学医学院附属同仁医院、南京医科大学附属南京医院、广州医科大学附属第一医院、徐州市儿童医院门急诊行血常规检测的抗凝静脉血标本201例,混匀后用CDR模式检测采血后第0、5、15、30分钟的PLT;制备这4个时间部分标本的血涂片并染色,观察血涂片两侧、片尾、海岸线处PLT聚集情况。结果与第30分钟比较,阻抗法第0、5、15分钟的PLT计数均值偏差分别为6.92%、3.28%、1.65%;optical platelet,光学法第0、5、15分钟的PLT计数均值偏差分别为0.76%、0.35%、0.03%。采血后第0、5、15分钟的光学法PLT计数显著高于阻抗法PLT计数(P<0.001);采血后第30分钟,2种方法PLT计数差异无统计学意义(P=0.686)。结论采血后第0 min,阻抗法PLT计数由可逆性聚集导致的假性减低幅度>5%,但可被光学法纠正。Objective To investigate whether the depolymerization effect of optical platelet(PLT)count on ethylenediaminetetraacetic acid-dependent pseudothrombocytopenia(EDTA-PTCR)applicable to reversible PLT aggregation that occurs immediately after anticoagulant blood collection.Methods A total of 201 venous blood samples collected from December 2021 to August 2022 at the outpatient and emergency departments of Tongren Hospital of Shanghai Jiao Tong University School of Medicine,Nanjing Hospital Affiliated to Nanjing Medical University,Guangzhou First Hospital Affiliated to Guangzhou Medical University and Xuzhou Children's Hospital.PLT counting was performed at 0,5,15 and 30 min after blood collection using CDR mode.Blood smear preparation and staining were performed at the above time points for partial samples.PLT aggregation was evaluated at the both sides,the tail and the edge of blood smears.Results Comparing with impedance PLT count at 30 min,the mean deviations at 0,5 and 15 min were 6.92%,3.28%and 1.65%,respectively.Comparing with optical PLT counts at 30 min,the mean deviations at 0,5 and 15 min were 0.76%,0.35%and 0.03%,respectively.PLT counts were higher in optical method compared to impedance method at 0,5 and 15 min after blood collection(P<0.001).No statistical significance was found between the 2 methods at 30 min after blood collection(P=0.686).Conclusions The mean deviation of impedance PLT count at 0 min after blood collection is>5%caused by reversible PLT aggregation,which could be corrected by optical method.

关 键 词：血小板 光学法血小板计数 阻抗法血小板计数 血小板聚集 血小板假性减低 

分 类 号：R446.11[医药卫生—诊断学]