一株内切葡聚糖酶产生菌株的分离鉴定及其产酶条件优化  

Isolation and identification of an endoglucanase producing strain and optimization of its enzymatic production conditions

作　　者：赵思卡 许倍滔 冯喆 王力 张建国 李晓华[1,2] ZHAO Sika;XU Beitao;FENG Zhe;WANG Li;ZHANG Jianguo;LI Xiaohua(South-Central Minzu University,College of Life Sciences,Wuhan 430074,China;South-Central Minzu University,Hubei Provincial Engineering and Technology Research Center for Resources and Utilization of Microbiology,Wuhan 430074,China;Jiangsu Shenli Ecological Agricultural Science and Technology Co Ltd,Yixing 214211,Jiangsu China)

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]中南民族大学微生物资源与利用湖北省工程技术研究中心,武汉430074 [3]江苏神力生态农业科技有限公司,江苏宜兴214211

出　　处：《中南民族大学学报（自然科学版）》2024年第1期24-31,共8页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(31070087);中央高校基本科研业务费专项资金资助项目(CZY19018);中南民族大学企业委托资助项目(HZY20042,HZY21015)。

摘　　要：从湖南省长沙县养殖场牛胃秸秆发酵物中分离获得了一株高产内切葡聚糖酶的菌株,命名为SCUEC7.通过菌落形态、生理生化特性及16S rDNA序列分析,鉴定SCUEC7菌株为枯草芽胞杆菌(Bacillus subtilis).当培养时间为12 h、pH值为6.0、培养温度为37°C、碳源为20.0 g·L^(-1)麦芽糖、氮源为15.0 g·L^(-1)胰蛋白胨、金属离子为1.5 g·L^(-1)的Mn^(2+)的条件下,枯草芽胞杆菌SCUEC7菌株菌体生长量和内切葡聚糖酶活性均较高.在单因素实验的基础上,响应面法分析显示:培养11.9 h,初始pH值为5.9,麦芽糖含量为19.8 g·L^(-1)时,预测最高酶活力为409.0 U·mL^(-1),内切葡聚糖酶的酶活力实测值与预测值之间有较高的拟合度.SCUEC7菌株产生内切葡聚糖酶活性较高,为其秸秆饲料中的应用奠定基础.A strain with high production of endoglucanase was selected from the straw fermentation of cattle stomach in Changsha County,Hunan Province,and was named SCUEC7.Based on the morphological observation,physiological and biochemical characteristics,and 16S rDNA sequence comparison analysis,SCUEC7 strain was identified as Bacillus subtilis.The culture conditions for the better growth of SCUEC7 strain were as follows:12 h of culture period,pH 6.0,37°C of culture temperature,20.0 g·L^(-1)maltose as carbon source,15.0 g·L^(-1) tryptone as nitrogen source,1.5 g·L^(-1) Mn^(2+) as metal ion.Response surface methodology analysis showed that the highest predicted enzyme activity was 409.0 U·mL^(-1) when the initial pH value was 5.9,maltose content was 19.8 g·L^(-1),and culture period was 11.8 h.There was a high degree of fit between the measured and predicted values of endoglucanase activity.SCUEC7 strain has a strong ability to produce endoglucanase,which can provide a basis for the development of animal feed.

关 键 词：枯草芽胞杆菌SCUEC7菌株 内切葡聚糖酶 产酶条件优化 响应面法优化 

分 类 号：Q93[生物学—微生物学]