Immobilization and characterization of the Lactarius deliciosus mycelia-embedded polylysine-alginate beads and their decolorization performance  

作　　者：Yiwen Jin Jie Yuan Caixia Liu Jiacheng Sun Youbin Liu Zhifeng Ding Qingxi Wu 

机构地区：[1]School of Life Sciences,Anhui University,Hefei 230601,China [2]Key Laboratory of Eco-engineering and Biotechnology of Anhui Province and Anhui Key Laboratory of Modern Biomanufacturing,Hefei 230601,China [3]Department of Chemistry,The University of Western Ontario,Ontario N6A 5B7,Canada

出　　处：《Frontiers of Chemical Science and Engineering》2023年第12期2001-2013,共13页化学科学与工程前沿（英文版）

基　　金：supported by the Natural Science Foundation of Anhui Province,China(Grant No.2208085MB32);the Anhui Provincial Program on Key Research and Development Project,China(Grant No.202004a06020021);the Key Research Program on Natural Science of Anhui Higher Education,China(Grant No.KJ2020A0049);the National Natural Science Foundation of China(Grant No.21606002);the Undergraduate Research Training Program for Innovation(Grant No.202210357050).

摘　　要：Liquid fermented fungal mycelia with decolorization capability have potential applications in scale-up. In this work, the Lactarius deliciosus mycelia were immobilized on ε-polylysine-alginate beads, and the decolorization effects of ε-polylysine-alginate beads were demonstrated along with Coomassie brilliant blue G-250 as a model dye. Morphology observation confirmed the beads had an exterior film and interior capsule with honeycomb microstructures suitable for mycelia growth. It was manifested that the maximum decolorization efficiency for mycelia was 98.5% at a removal rate of 0.68 mg·L^(-1)·h after 3 days. In comparison, the decolorization efficiency of the immobilized mycelia reached the maximum value of 97.3% at a removal rate of 6.1 mg·L^(-1)·h after 8 h. The enzyme activities of lignin peroxidase and laccase tested in the immobilized mycelia were significantly higher than in that of the free ones, such as the lignin peroxidase had the highest enzyme activity of 77.6 ± 7.4 U·L^(-1) in the former, while of 27.4 ± 8.7 U·L^(-1) in the latter. The immobilization of L. deliciosus mycelia could improve the decolorization of Coomassie brilliant blue G-250 efficiently. The prepared ε-polylysine-alginate beads embedded with L. deliciosus mycelia have very good reusability and a great potential in decolorizing analog dyes.

关 键 词：Lactarius deliciosus mycelia IMMOBILIZATION DECOLORIZATION polylysine-alginate beads Coomassie brilliant blue G-250 

分 类 号：O62[理学—有机化学]