CCL3、sCD40L在急性肺栓塞患者血清中的表达及与疾病严重程度和预后的关系  

Expression of CCL3 and sCD40L in serum of acute pulmonary embolism patients and their relationship with disease severity and prognosis

作　　者：杨帅 张亚楠 董岩 豆亚静 范耀东[2] YANG Shuai;ZHANG Yanan;DONG Yan;DOU Yajing;FAN Yaodong(Civil Aviation General Hospital,Beijing 100123,China;Yunnan Cancer Hospital,Kunming 650118,Yunnan,China)

机构地区：[1]民航总医院,北京100123 [2]云南省肿瘤医院,云南昆明650118

出　　处：《武汉大学学报（医学版）》2023年第12期1501-1505,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:81960455)。

摘　　要：目的:探索趋化因子3(CCL3)、可溶性白细胞分化抗原CD40配体(sCD40L)在急性肺栓塞(APE)患者血清中的表达及与疾病严重程度和预后的关系。方法:选取2020年1月—2022年3月在民航总医院急诊科确诊的APE患者126例进入APE组,按照疾病的严重程度分为低危组(56例)、中危组(40例)、高危组(30例),根据APE患者出院后随访6个月的生存情况分为存活组(92例)和死亡组(34例)。同期选择在我院体检健康的志愿者110例进入对照组。采用酶联免疫吸附法(ELISA)检测血清中CCL3、sCD40L表达水平。采用Pearson法分析血清CCL3、sCD40L表达水平的相关性;多因素Logistic回归分析影响APE患者预后的因素。结果:与对照组相比,APE组血清CCL3、sCD40L表达水平均显著升高(P<0.05)。高危组CCL3、sCD40L的表达水平显著高于中危组及低危组,中危组显著高于低危组(P<0.05)。相关性分析显示,APE患者血清中CCL3和sCD40L表达水平呈正相关(P<0.05)。与死亡组相比,存活组CCL3、sCD40L表达水平显著降低(P<0.05)。Logistic回归分析显示,吸烟史、收缩压、舒张压、CCL3和sCD40L表达水平是影响APE患者预后的因素(P<0.05)。结论:APE患者血清中CCL3和sCD40L表达水平呈异常升高,与疾病严重程度有关,两者是影响APE患者预后的因素。Objective:To investigate the expression of chemokine(C-C motif)ligand 3(CCL3)and the solu-ble cluster of differentiation 40 ligands(sCD40L)in the serum of patients with acute pulmonary embo-lism(APE)and their relationship with the severity and prognosis of the disease.Methods:A total of 126 patients with APE diagnosed in the emergency department of Civil Aviation General Hospital in Beijing from January 2020 to March 2022 were selected as the APE group.According to the severity of the disease,they were divided into a low-risk group(56 cases),a medium-risk group(40 cases)and a high-risk group(30 cases).All the APE patients were also divided into a survival group(92 cases)and a death group(34 cases)according to their six-month survival after discharge.At the same time,110 healthy volunteers who had physical examinations were selected as the control group.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expression of CCL3 and sCD40L in se-rum.Pearson method was used to analyze the correlation between the expression levels of serum CCL3 and sCD40L;multivariate logistic regression analysis was used to analyze the factors influenc-ing the prognosis of APE patients.Results:Compared with those in the control group,serum CCL3 and sCD40L levels in the APE group were higher(P<0.05).The expression levels of CCL3 and sCD40L in the high-risk group were higher than those in the medium-risk and low-risk groups,and those in the medium-risk group were higher than those in the low-risk group(P<0.05).Correlation analysis showed that the expression levels of CCL3 and sCD40L in the serum of APE patients were positively correlated(P<0.05).Compared with the death group,the expression levels of CCL3 and sCD40L in the survival group decreased obviously(P<0.05).Logistic regression analysis showed that smoking history,systolic blood pressure,diastolic blood pressure,and the expression levels of CCL3 and sCD40L were factors affecting the prognosis of APE patients(P<0.05).Conclusion:The expression level of CCL3 and sCD40L in the

关 键 词：急性肺栓塞 趋化因子3 可溶性白细胞分化抗原CD40配体 预后 

分 类 号：R563.5[医药卫生—呼吸系统]