人参多糖调控Wnt3/β-catenin/Runx2信号通路改善去卵巢大鼠骨质疏松的作用  

Effect of ginseng polysaccharide on relieving osteoporosis in ovariectomized rats by regulation of Wnt3/β-catenin/Runx2 signaling pathway

作　　者：李高峰[1] 王艺昧[2] 李光淳[1] 张扬[1] 王博 杜彦辉 桑平[1] LI Gaofeng;WANG Yimei;LI Guangchun;ZHANG Yang;WANG Bo;DU Yanhui;SANG Ping(The Second Department of Traumatic Bone Disease,Jilin Provincial People’s Hospital,Changchun 130021;Department of Pathology,the First Clinical Hospital of Jilin University,Changchun 130021,China)

机构地区：[1]吉林省人民医院创伤骨病二科,吉林长春130021 [2]吉林大学第一临床医院病理科,吉林长春130021

出　　处：《中国骨质疏松杂志》2023年第12期1730-1736,共7页Chinese Journal of Osteoporosis

基　　金：吉林省科技发展计划项目(YDZJ202201ZYS228)。

摘　　要：目的探究人参多糖改善去卵巢大鼠骨质疏松的作用及对Wnt3/β-catenin/Runx2信号通路的影响。方法将大鼠分为假手术组、模型组、人参多糖低、高剂量组(100、200 mg/kg)及阿仑膦酸钠维生素D3组(6.25 mg/kg),每组10只,采用去卵巢法建立骨质疏松症模型。连续干预12周,记录体质量,观察骨组织病理形态,检测骨密度、骨生物力学、血清骨代谢及生化指标,同时测定骨组织Wnt3、β-catenin及Runx2表达。结果与模型组比较,人参多糖低、高剂量组大鼠体质量明显降低(P<0.01),骨组织病理形态减轻,骨密度、刚度、最大应力及最大载荷均明显增加(P<0.05,P<0.01);血清TRAP-5b含量明显降低(P<0.05,P<0.01),而BALP、OC、OPG及P^(3+)、Ca^(2+)含量明显升高(P<0.05,P<0.01);骨组织Wnt3、β-catenin及Runx2表达均明显增加(P<0.05,P<0.01)。结论人参多糖具有改善去卵巢大鼠骨质疏松的作用,其机制可能与激活Wnt3/β-catenin/Runx2信号通路有关。Objective To explore the effect of ginseng polysaccharide on relieving osteoporosis and Wnt3/β-catenin/Runx2 signaling pathway in ovariectomized rats.Methods Rats were divided into five groups,sham operation group,model group,low-dose and high-dose ginseng polysaccharide groups(100 and 200 mg/kg),and alendronate sodium vitamin D3 group(6.25 mg/kg),with 10 rats in each group.Osteoporosis model was established using ovariectomy.After continuous intervention for 12 weeks,body mass was recorded.The histopathologic morphology of the bone was observed.Bone mineral density,bone biomechanical indexes,serum bone metabolism,and biochemical indexes were detected.Expressions of Wnt3,β-catenin,and Runx2 in the bone tissue were measured.Results Compared to those in the model group,the body mass of rats in low-dose and high-dose ginseng polysaccharide groups decreased significantly(P<0.01),the histopathologic morphology of bone was alleviated,and bone mineral density,stiffness,and maximum stress and maximum load increased significantly(P<0.05,P<0.01).Serum TRAP-5b content decreased significantly(P<0.05,P<0.01).The contents of BALP,OC,OPG,P^(3+),and Ca^(2+)increased significantly(P<0.05,P<0.01).The expressions of Wnt3,β-catenin,and Runx2 in the bone tissue increased significantly(P<0.05,P<0.01).Conclusion Ginseng polysaccharide relieves osteoporosis in ovariectomized rats.Its mechanism may be related to the activation of Wnt3/β-catenin/Runx2 signaling pathway.

关 键 词：人参多糖 骨质疏松 去卵巢大鼠 Wnt3/β-catenin/Runx2信号通路 

分 类 号：R274.9[医药卫生—中西医结合] R285.5[医药卫生—中医骨伤科学] R-332[医药卫生—中医学]