lncRNA NEAT1/SFPQ/RUNX2轴研究P-15对软骨损伤的保护作用  

LncRNA NEAT1/SFPQ/RUNX2 axis study on the protective effect of P-15 on cartilage injury

作　　者：邓长弓[1] 李源力 聂君兰 李洪 DENG Changgong;LI Yuanli;NIE Junlan;LI Hong(North Sichuan Medical College,Nanchong 637000,China;The Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]川北医学院,四川南充637000 [2]川北医学院附属医院,四川南充637000

出　　处：《中国骨质疏松杂志》2023年第12期1750-1755,共6页Chinese Journal of Osteoporosis

基　　金：2022年度川北医学院附属医院科研发展计划项目(2022JC008)。

摘　　要：目的探究P-15对人骨关节炎软骨细胞(HC-OA)的保护作用及与lncRNA NEAT1/SFPQ/RUNX2轴的关系。方法分离HC-OA胞核、胞质RNA,RT-qPCR检测lncRNA NEAT1在细胞中的分布;P-15处理HC-OA细胞,转染siRNA NC和siRNA SFPQ后,RT-qPCR检测lncRNA NEAT1表达水平;流式细胞仪检测细胞凋亡;Western blot检测RUNX2、SFPQ、Beclin-1、LC3B和P62表达水平。结果lncRNA NEAT1同时存在于HC-OA胞质和胞核,且更多分布于细胞核;P-15可降低胞内lncRNA NEAT1水平(P<0.05),抑制骨关节炎软骨细胞凋亡(P<0.05);P-15可通过调控lncRNA NEAT1/SFPQ/RUNX2轴上调SFPQ表达和抑制RUNX2表达(P<0.01);P-15调控lncRNA NEAT1/SFPQ/RUNX2轴促进软骨细胞发生自噬(P<0.05)。结论P-15可调控lncRNA NEAT1/SFPQ/RUNX2轴抑制HC-OA凋亡并促进自噬,保护软骨细胞免受损伤。Objective To investigate the protective effect of P-15 on human osteoarthritic chondrocytes(HC-OA)and its relationship with lncRNA NEAT1/SFPQ/RUNX2 axis.Methods Nuclear and cytoplasmic RNAs of HC-OA were isolated,and the distribution of lncRNA NEAT1 in HC-OA cells was detected by RT-qPCR;HC-OA cells were treated with P-15 and transfected with siRNA NC and siRNA SFPQ,and the expression levels of lncRNA NEAT1 were detected by RT-qPCR;apoptosis was detected by flow cytometry;and the expression levels of RUNX2,SFPQ,Beclin-1,LC3B and P62 were detected by Western blot.Results lncRNA NEAT1 was present in both the cytoplasm and nucleus of HC-OA,and was more distributed in the nucleus;P-15 could reduce the intracellular lncRNA NEAT1 level(P<0.05)and inhibit osteoarthritis chondrocyte apoptosis(P<0.05);P-15 could up-regulate SFPQ expression and inhibit RUNX2 expression by regulating the lncRNA NEAT1/SFPQ/RUNX2 axis(P<0.01);P-15 regulated the lncRNA NEAT1/SFPQ/RUNX2 axis to promote autophagy in chondrocytes(P<0.05).Conclusion P-15 can regulate lncRNA NEAT1/SFPQ/RUNX2 axis to inhibit HC-OA apoptosis and promote autophagy,and protect chondrocytes from injury.

关 键 词：P-15肽 lncRNA NEAT1/SFPQ/RUNX2轴 人骨关节炎软骨细胞 凋亡 自噬 

分 类 号：R392.5[医药卫生—免疫学]