流式细胞分析仪在细胞因子检验中的应用价值  

Application Value of Flow Cytometry in Cytokine Detection

作　　者：董一波 沈煜轩 姚沈明 DONG Yibo;SHEN Yuxuan;YAO Shenming(Department of Laboratory,Jiangsu Shengze Hospital,Shengze,Jiangsu Province,215228 China)

机构地区：[1]江苏盛泽医院检验科,江苏盛泽215228

出　　处：《系统医学》2023年第20期40-43,共4页Systems Medicine

摘　　要：目的分析流式细胞分析仪在细胞因子检验中应用的价值。方法选取2023年2—5月在江苏盛泽医院接受健康体检者和门诊住院患者(共112例)血浆标本,运用流式细胞分析仪分析血浆标本的8项细胞因子精密度、准确度和线性范围验证实验结果。结果低值和高值连续5 d检测结果变异系数(coefficient of variability,CV)均≤15%。低值和高值连续5 d检测结果准确度均符合(偏差≤±15%)范围。8项细胞因子检测试剂盒在流式细胞分析仪上检测结果均在范围内呈线性,满足准确度(r2≥0.98)的标准范围,r2分别为0.9988、1.0000、1.0000、0.9998、1.0000、0.9994、0.9995、0.9843。结论流式细胞分析仪在细胞因子检测中的应用的准确度、可靠性和重复性较好,能满足临床需求,可广泛应用于实验室相关检测当中。Objective To analyze the application value of flow cytometry in cytokine detection.Methods Plasma samples were collected from 112 patients(in total)who received physical examination in Shengze Hospital from February to May 2023.The accuracy,accuracy and linear range of 8 cytokines in plasma samples were analyzed by flow cytometry to verify the experimental results.Results Coefficient of variance(CV)values of both low and high values for 5 dconsecutive days were≤15%.The accuracy of low and high values for 5 d continuous detection was in the range of(deviation≤±15%).The results of 8 cytokine detection kits on flow cytometry analyzer were linear in the range,meeting the standard range of accuracy(r2≥0.98),r2 was 0.9988,1.0000,1.0000,0.9998,1.0000,0.9994,0.9995,0.9843,respectively.Conclusion The accuracy,reliability and repeatability of flow cytometry in the detection of cytokines are good,which can meet the clinical needs,and can be widely used in laboratory related detection.

关 键 词：流式细胞分析仪 细胞因子 临床检验 

分 类 号：R4[医药卫生—临床医学]