Silencing of the Nonspecific Phospholipase C6(NPC6)Gene Induces Ricinoleic Acid Accumulation in Castor Seeds  

作　　者：Yong Zhao Lili Li Rui Luo Mu Peng Tongtong Jiang Mingda Yin Yanpeng Wen Zihan Wang Fenglan Huang Fanjuan Meng 

机构地区：[1]College of Life Science and Food,Inner Mongolia Minzu University,Tongliao,028000,China [2]College of Life Science,Baicheng Normal University,Baicheng,137000,China [3]College of Life Science,Northeast Forestry University,Harbin,150040,China [4]Key Laboratory of Castor Breeding of the State Ethnic Affairs Commission,Inner Mongolia Minzu University,Tongliao,028043,China [5]Inner Mongolia Industrial Engineering Research Center of Universities for Castor,Inner Mongolia Minzu University,Tongliao,028043,China [6]Inner Mongolia Key Laboratory of Castor Breeding and Comprehensive Utilization,Inner Mongolia Minzu University,Tongliao,028000,China [7]Inner Mongolia Engineering Research Center of Industrial Technology Innovation of Castor,Inner Mongolia Minzu University,Tongliao,028000,China

出　　处：《Phyton-International Journal of Experimental Botany》2023年第12期3237-3250,共14页国际实验植物学杂志（英文）

基　　金：supported by the following agencies:Natural Science Foundation of Jilin Province (YDZJ202201ZYTS453);Scientific Research Project of the Jilin Provincial Department of Education (JJKH20220010KJ);supported by Program for Innovative Research Team of Baicheng Normal University,National Natural Science Foundation of China (31860071);Inner Mongolia Autonomous Region Natural Science Foundation Project (2021MS03008);Inner Mongolia Autonomous Region Grassland Talent Innovation Team (2022);2022 Basic Scientific Research Business Cost Project of Universities Directly under the Autonomous Region (237);Open Fund Project of Inner Mongolia Castor Industry Collaborative Innovation Center (MDK2021011,MDK2022014,MDK2022008,MDK2021008,MDK2022009);Fundamental Research Funds for Universities Directly under the Autonomous Region in 2023 of Inner Mongolia University for Nationalities (225,227,243,244);New Agricultural Science Research and Reform Practice Project of the Ministry of Education (2020114)。

摘　　要：Castor,scientifically known as Ricinus communis L.,is among the top ten oil crops globally.It is considered a renewable resource and is commonly referred to as‘green oil’.Castor seeds contain castor oil as their main component,which is predominantly composed of ricinoleic acid.This study utilized RNAi technology to silence the NPC6 gene in NO.2129 castor,resulting in the creation of mutant plants L1 and L2.The weight of 100 dry seed kernels from L1 and L2 exceeds that from NO.2129.The crude fat and ricinoleic acid levels of L1 and L2 were higher than those of NO.2129 at various developmental stages.In the proteomics analysis of 60-day-old castor seeds,a total of 21 differentially expressed proteins were identified,out of which 19 were successfully recognized.Eleven of the differentially expressed proteins identified were legumins,which play a crucial role in nutrient storage within the seed.Silencing the NPC6 gene results in the accumulation of ricinoleic acid in castor seeds.The findings of this study not only enhance our knowledge of NPC6’s role in regulating castor seed oil synthesis but also offer fresh perspectives for investigating oil synthesis and accumulation in other plant species.

关 键 词：Ricinus communis L ricinoleic acid NPC6 RNAI 

分 类 号：Q94[生物学—植物学]